Abstract
Rat electrophysiological measurements are widely used in experimental protocols to study heart diseases, drug assessment, and their interaction. However, commercially employed rat ECG recording systems demand a high cost for implantation due to the increased signal conditioning complexity and experimental arrangements compared to the ECG from a human subject. These issues preclude its greater use for experimental research in emerging countries. In this perspective, we designed and implemented a low-cost, simplified system for recording the cardiac electric activity in rats, meeting the minimum specifications for suitable data processing. The hardware developed in the system consisted of four blocks, including conditioning, conversion, transmission, and system power supply; the acquired signals are stored and processed on a personal computer. Our system demonstrated capacity for recording ECG signals successfully with low-noise and minimum requirements. Also, data analysis showed that with simple filtering was possible to identify the R peak, allowing the further analysis of ECG rhythmicity and morphology and calculation of heart rate variability indexes. Therefore, we concluded that this low-cost system proved to be adequate for ECG recording in rats. Nonetheless, we encouraged further work to better assessment in the different experimental protocols and other rodents.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Biswal D, Uvanesh K, Champaty B, Ray SS, Pal K (2014) Development of an ambulatory universal biopotential recording device. In: International Conference on Control, Instrumentation, Communication and Computational Technologies, pp 1124–1129. IEEE. https://doi.org/10.1109/ICCICCT.2014.6993130
Serhani MA, El Kassabi HT, Ismail H, Navaz AN (2020) ECG monitoring systems: review, architecture, processes, and key challenges. Sensors (Basel) 20:1796
Wang T-W, Lin S-F (2020) Non-contact capacitive sensing for ECG recording in small animals. Sci Technol
Niemeyer JE (2016) Telemetry for small animal physiology. Lab Anim 45:255–257
Task Force of the European Society of Cardiology & The North American Society of Pacing and Electrophysiology (1996) Heart rate variability: standards of measurement, physiological interpretation, and clinical use. Eur Heart J 17:354–381
Bolanos M, Nazeran H, Haltiwanger E (2006) Comparison of heart rate variability signal features derived from electrocardiography and photoplethysmography in healthy individuals. In: Engineering in medicine and biology society. IEEE, pp 4289–4294
Nazeran H (2006) Electrocardiography, computers in. In: Webster JG (ed) Encyclopedia of medical devices and instrumentation. Wiley, pp 34–52
Kwon O et al (2018) Electrocardiogram sampling frequency range acceptable for heart rate variability analysis. Heal Inf Res 24:198–206
Ziemssen T, Gasch J, Ruediger H (2008) Influence of ECG sampling frequency on spectral analysis of RR intervals and baroreflex sensitivity using the EUROBAVAR data set. J Clin Monit Comput 22:159–168
Arini PD, Liberczuk S, Mendieta JG, Santa Mariá M, Bertrán GC (2018) Electrocardiogram delineation in a Wistar rat experimental model. Comput Math Methods Med 2018:2185378
Pereira-Junior PP, Marocolo M, Rodrigues FP, Medei E, Nascimento JHM (2010) Noninvasive method for electrocardiogram recording in conscious rats: feasibility for heart rate variability analysis. An Acad Bras Cienc 82:431–437
Botelho AFM et al (2019) Non-invasive ECG recording and QT interval correction assessment in anesthetized rats and mice. Pesq Vet Bras 39:409–415
Nascimento RS, Fiorin FS, Santos ARS, Royes LFF, Marques JLB (2020) A new approach for ECG recording in rats: an autonomic nervous system analysis. In: Proceedings of the 5th Brazilian Technology Symposium, Smart Innovation, Systems and Technologies, vol 202 Springer
Jafarinezhad Z, Rafati A, Ketabchi F, Noorafshan A, Karbalay-Doust S (2019) Cardioprotective effects of curcumin and carvacrol in doxorubicin-treated rats: Stereological study. Food Sci Nutr 7:3581–3588
Kalkan F et al (2018) Protective and therapeutic effects of dexpanthenol on isoproterenol-induced cardiac damage in rats. J Cell Biochem 119:7479–7489
Acknowledgments
The authors gratefully acknowledge the student fellowships from the National Council for Scientific and Technological Development (CNPq) (to RSN) and the Coordination of Superior Level Staff Improvement (CAPES) (to FSF). JLBM is a grantee of the CNPq research productivity fellowship.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this paper
Cite this paper
do Nascimento, R.S., da Silva Fiorin, F., Marques, J.L.B. (2021). Design and Implementation of an ECG Recording System for in Vivo Experimentation in Rats. In: Iano, Y., Saotome, O., Kemper, G., Mendes de Seixas, A.C., Gomes de Oliveira, G. (eds) Proceedings of the 6th Brazilian Technology Symposium (BTSym’20). BTSym 2020. Smart Innovation, Systems and Technologies, vol 233. Springer, Cham. https://doi.org/10.1007/978-3-030-75680-2_37
Download citation
DOI: https://doi.org/10.1007/978-3-030-75680-2_37
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-75679-6
Online ISBN: 978-3-030-75680-2
eBook Packages: EngineeringEngineering (R0)