Abstract
We have used cyanobacterium Synechococcus elongatus PCC 7942 as the model system and looked for the slow but temperature-compensated reaction functioning as the pacemaker of in vivo rhythms. The key reaction we focused was the ATP hydrolysis as slow as 12 ATP d−1 in the N-terminal half of the clock protein KaiC. This intra-molecular-scale slowness comes from structural regulations of steric hindrance, water molecules, and cis-to-trans peptide isomerization in KaiC, being related on one-to-one correspondence not only to the frequency of inter-molecular-scale rhythm of KaiA/KaiB/KaiC oscillator, but also to the frequency of cellular-scale rhythms.
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Acknowledgments
I would like to thank Drs. T. Kondo (Graduate School of Science, Nagoya University, Japan), E. Yamashita (Institute for Protein Research, Osaka University, Japan), A. Mukaiyama, Y. Furuike, D. Ouyang, D. Simon, T. Mori, and S. Saito (Institute for Molecular Science, NINS, Japan) for their contributions to the study of the cyanobacterial circadian clock system.
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Akiyama, S. (2021). Reasons for Seeking Information on the Molecular Structure and Dynamics of Circadian Clock Components in Cyanobacteria. In: Johnson, C.H., Rust, M.J. (eds) Circadian Rhythms in Bacteria and Microbiomes. Springer, Cham. https://doi.org/10.1007/978-3-030-72158-9_8
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DOI: https://doi.org/10.1007/978-3-030-72158-9_8
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