Abstract
This study investigates the effect of the nitrided layer on antibacterial properties of biomedical stainless steel, grade 316L. The surfaces were sanded #1200 mesh papers, subsequently plasma-nitrided at a temperature of 400 °C for 2 h, which resulted in a 4.5 μm thick layer. The characterization was made using optical and scanning electron (SEM) microscopies, energy dispersive spectrometry (EDS) X-ray diffraction, and Vickers hardness testing. The antibacterial characteristic of the untreated steel and the nitrided layer was evaluated using Gram-negative Escherichia coli ATCC8739, according to Japanese International Standard (JIS) no. Z2801:2000. A single γ-phase layer was formed, supersaturated in nitrogen, increasing the surface hardness significantly by 35%. The bacterial test demonstrated that the nitrided layer presented suitable antibacterial characteristics.
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References
Olzon-Dionysio M, Campos M, Higa OZ et al (2013) Investigating the correlation between some of the properties of plasma nitrided AISI 316L stainless steel. Mater Res 16(5):1052–1057. https://doi.org/10.1590/S1516-14392013005000081
Mendes AF, Scheuer CJ, Joanidis IL et al (2014) Low-temperature plasma nitriding of sintered PIM 316L austenitic stainless steel. Mater Res 17(Supl.1):100–8. https://doi.org/10.1590/S1516-14392014005000064.
Reis RF, Maliska AM, Borges PC (2011) Nitrogen surface enrichment of austenitic stainless steel ISO 5832–1. J Mater Sci 46(3):846–854. https://doi.org/10.1007/s10853-010-4827-3
Bernardelli EA, Borges PC, Fontana LC et al (2010) Role of plasma nitriding temperature and time in the corrosion behaviour and microstructure evolution of 15–5 PH stainless steel. Kovové Materiály 48(2):105–116. https://doi.org/10.4149/km_2010_2_105
Li-Hsiang L, Shih-Chung C, Ching-Zong W et al (2011) Microstructure and antibacterial properties of microwave plasma nitrided layers on biomedical stainless steels. App Surf Sci 257:7375–7380. https://doi.org/10.1016/j.apsusc.2011.01.065
Dong Y, Li X, Bell T et al (2010) Surface microstructure and antibacterial property of an active-screen plasma alloyed austenitic stainless steel surface with Cu and N. Bio Mater 5:054105. https://doi.org/10.1088/1748-6041/5/5/054105
Sphair AC (2017) Nitretação por plasma de aço inoxidável austenítico com fluxo pulsado de nitrogênio [dissertação]. Curitiba: Programa de Pós-graduação em Engenharia Mecânica e de Materiais, Universidade Tecnológica Federal do Paraná
Öztürk O, Williamson DL (1995) Phase and composition depth distribution analysis of low energy, high flux N implanted stainless steel. J Appl Phys 77:3839–3850. https://doi.org/10.1063/1.358561
Reis RF, Silva PGHM, Villanova RL et al (2020) Plasma nitriding of ISO 5832-1 stainless steel with intermittent nitrogen flow at 450 °C. Mater Res 23(1):e20190501. https://doi.org/10.1590/1980-5373-MR-2019-0501
Hung CF, Wu CZ, Lee KL et al (2012) The effect of nitrided layer on antibacterial properties for biomedical stainless steel. Phys Procedia 32:914–919. https://doi.org/10.1016/j.phpro.2012.03.656
Ou KL, Chou HH, Liu CM et al (2011) Surface modification of austenitic stainless steel with plasma nitriding for biomedical applications. Surf Coat Technol 206:1142–1145. https://doi.org/10.1016/j.surfcoat.2011.08.001
Li J, He X, Zhang G et al (2018) Effect of nitriding on antibacterial performance of 8Cr17 and 4Cr13 martensitic stainless steel. Mater Perform Charact 7(1):70–77. https://doi.org/10.1520/MPC20170058
Zagonel LF, Bettini J, Basso RLO et al (2012) Nanosized precipitates in H13 tool steel low temperature plasma nitriding. Surf Coat Technol 207:72–78. https://doi.org/10.1016/j.surfcoat.2012.05.081
Acknowledgements
The authors thank the Multi-User Center for Materials Characterization (CMCM) of the UTFPR for XRD and SEM analysis, and Professor G. Pintaude for his revisions and suggestions.
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The authors declare that they have no conflict of interest.
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Benegra, M., Couto, G.H., Bernardelli, E.A. (2022). The Effect of Nitrided Layer on Antibacterial Properties of Biomedical 316L Stainless Steel. In: Bastos-Filho, T.F., de Oliveira Caldeira, E.M., Frizera-Neto, A. (eds) XXVII Brazilian Congress on Biomedical Engineering. CBEB 2020. IFMBE Proceedings, vol 83. Springer, Cham. https://doi.org/10.1007/978-3-030-70601-2_21
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DOI: https://doi.org/10.1007/978-3-030-70601-2_21
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