What You Will Learn in This Chapter
This chapter aims to convey a tangible impression of how cell culture procedures affect the cultured cells. Many standard cell culture procedures have been developed decades ago and are still being used routinely. However, they may to some extent be harmful to the cultured cells and alter phenotype, functional characteristics, and potency as compared to the same cell type residing in the body. This is of crucial relevance during translational development of cell-based therapies, at which multipotent mesenchymal stromal cell (MSC) play a major role. Here we use the example of MSC isolation and culture to illustrate critical aspects of cell culture procedures on a theoretical as well as practical level. Finally, this chapter culminates in an unconventional cartoon style illustration of the cellular perspective. Imagine what life might be like in a cell culture dish.
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Burk, J. (2021). A View from the Cellular Perspective. In: Kasper, C., Egger, D., Lavrentieva, A. (eds) Basic Concepts on 3D Cell Culture . Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-030-66749-8_3
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