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Proteomics and Biomarker Identification in Improved Sperm Motility Parameters After 4 h of Ejaculatory Abstinence

  • Conference paper
  • First Online:
XIIIth International Symposium on Spermatology

Abstract

Various studies have sought to determine the optimal abstinence period after which semen samples should be collected, with many contradictory results reported. Several factors influence the semen microenvironment, and thus potentially sperm parameters. In this study, we focused on the secretions of the prostate, seminal vesicles and the epididymis. Semen samples were obtained from healthy normozoospermic males (n = 16) after 4 day and 4 h periods of ejaculatory abstinence (EA), and standard semen analysis was performed using computer-aided sperm analysis, whereas seminal plasma citric acid, neutral alpha-glucosidase and fructose concentrations were measured using assay kits. Proteomic analyses of seminal plasma proteins were performed using LC-MS/MS. There were significant decreases in total sperm count (P < 0.001), sperm concentration (P < 0.05) and semen volume (P < 0.05) after 4 h compared with 4 days ejaculatory abstinence. Furthermore, increases were observed in total sperm motility (P < 0.05), sperm progressive motility (P < 0.01), average path velocity (P < 0.001) and curvilinear velocity (P < 0.05) after a 4 h abstinence period, accompanied by significant reductions in citric acid (P < 0.05), neutral alpha-glucosidase (P < 0.01) and fructose (P < 0.01) concentrations. In addition, due to the decreased number of spermatozoa, these concentrations translated to a significant decrease in fructose (P < 0.05) per spermatozoon. A total of 2889 proteins were identified, with 24 proteins differentially expressed. These proteins have various roles in cellular processes and may influence the improvement of sperm motility after 4 h of EA. All these factors present in the seminal plasma indicate towards an intrinsic mechanism capitalising on alternative sources of energy for increased metabolic function and subsequent sperm motility.

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References

  • Aebersold R, Mann M (2003) Mass spectrometry-based proteomics. Nature 422(6928):198

    Article  CAS  PubMed  Google Scholar 

  • Agarwal A, Gupta S, Du Plessis S, Sharma R, Esteves SC, Cirenza C, Eliwa J, Al-Najjar W, Kumaresan D, Haroun N, Philby S, Sabanegh E (2016) Abstinence time and its impact on basic and advanced semen parameters. Urology 94:102–110

    Article  PubMed  Google Scholar 

  • Alipour H, Dardmeh F, Van Der Horst G, Manoharan G, Askeland A, Nielsena H (2015) Effect of short abstinence time on sperm motility parameters. Paper presented at the Final Programme, 18th World Congress of in Vitro Ferilization, Isivf

    Google Scholar 

  • Alipour H, Van Der Horst G, Christiansen O, Dardmeh F, Jørgensen N, Nielsen H, Hnida C (2017) Improved sperm kinematics in semen samples collected after 2 h versus 4–7 days of ejaculation abstinence. Hum Reprod 32(7):1364–1372

    Article  CAS  PubMed  Google Scholar 

  • Ashworth PJ, Harrison RA, Miller NG, Plummer JM, Watson PF (1994) Survival of ram spermatozoa at high dilution: protective effect of simple constituents of culture media as compared with seminal plasma. Reprod Fertil Dev 6(2):173–180

    Article  CAS  PubMed  Google Scholar 

  • Association, G. A. o. t. W. M (2014) World medical association declaration of Helsinki: ethical principles for medical research involving human subjects. J Am College Dentist 81(3):14

    Google Scholar 

  • Ayad BM, Van der Horst G, Du Plessis S (2018a) Revisiting the relationship between the ejaculatory abstinence period and semen characteristics. Int J Fertil Steril 11(4):238

    CAS  PubMed  Google Scholar 

  • Ayad BM, Van der Horst G, du Plessis S (2018b) Short abstinence: a potential strategy for the improvement of sperm quality. Middle East Fertil Soc J 23(1):37–43

    Article  Google Scholar 

  • Baas JW, Molan P, Shannon P (1983) Factors in seminal plasma of bulls that affect the viability and motility of spermatozoa. Reproduction 68(2):275–280

    Article  CAS  Google Scholar 

  • Baccetti B (1984) The human spermatozoon. In: Ultrastructure of reproduction. Springer, Berlin, pp 110–126

    Chapter  Google Scholar 

  • Bahadur G, Almossawi O, Zaid R, Ilahibuccus A, Al-Habib A, Muneer A, Okolo S (2016) Semen characteristics in consecutive ejaculates with short abstinence in subfertile males. Reprod BioMed Online 32(3):323–328

    Article  CAS  PubMed  Google Scholar 

  • Balk SP, Ko Y-J, Bubley GJ (2003) Biology of prostate-specific antigen. J Clin Oncol 21(2):383–391

    Article  CAS  PubMed  Google Scholar 

  • Batruch I, Lecker I, Kagedan D, Smith CR, Mullen BJ, Grober E, Lo KC, Diamandis EP, Jarvi KA (2011) Proteomic analysis of seminal plasma from normal volunteers and post-vasectomy patients identifies over 2000 proteins and candidate biomarkers of the urogenital system. J Proteome Res 10(3):941–953

    Article  CAS  PubMed  Google Scholar 

  • Bernardini A, Hozbor F, Sanchez E, Fornés M, Alberio R, Cesari A (2011) Conserved ram seminal plasma proteins bind to the sperm membrane and repair cryopreservation damage. Theriogenology 76(3):436–447

    Article  CAS  PubMed  Google Scholar 

  • Binz P-A, Hochstrasser DF, Appel RD (2003) Mass spectrometry-based proteomics: current status and potential use in clinical chemistry. Clin Chem Lab Med 41(12):1540–1551

    Article  CAS  PubMed  Google Scholar 

  • Bone W, Jones AR, Morin C, Nieschlag E, Cooper TG (2001) Susceptibility of glycolytic enzyme activity and motility of spermatozoa from rat, mouse, and human to inhibition by proven and putative chlorinated antifertility compounds in vitro. J Androl 22(3):464–470

    CAS  PubMed  Google Scholar 

  • Borges E Jr, Braga DPDAF, Setti AS (2018) Shorter ejaculatory abstinence interval and maternal endometrium exposure to seminal plasma as tools to improve pregnancy rate in patients undergoing intracytoplasmic sperm injection cycles. JBRA Assist Reprod 22(3):160

    PubMed  Google Scholar 

  • Caballero I, Parrilla I, Almiñana C, Del Olmo D, Roca J, Martínez E, Vázquez J (2012) Seminal plasma proteins as modulators of the sperm function and their application in sperm biotechnologies. Reprod Domest Anim 47:12–21

    Article  PubMed  Google Scholar 

  • Canale D, Bartelloni M, Negroni A, Meschini P, Izzo P, Bianchi B, Menchini‐Fabris G (1986) Zinc in human semen. Int J Androl 9(6):477–480

    Article  CAS  PubMed  Google Scholar 

  • Cao W-L, Wang Y-X, Xiang Z-Q, Li Z (2003) Cryopreservation-induced decrease in heat-shock protein 90 in human spermatozoa and its mechanism. Asian J Androl 5(1):43–46

    CAS  PubMed  Google Scholar 

  • Comar VA, Petersen CG, Mauri AL, Mattila M, Vagnini LD, Renzi A, Petersen B, Nicoletti A, Dieamant F, Oliveira JBAJJAR (2017) Influence of the abstinence period on human sperm quality: analysis of 2,458 semen samples. JBRA Assist Reprod 21(4):306

    PubMed  PubMed Central  Google Scholar 

  • Cooper T, Yeung C, Nashan D, Jockenhövel F, Nieschlag E (1990) Improvement in the assessment of human epididymal function by the use of inhibitors in the assay of α‐glucosidase in seminal plasma. Int J Androl 13(4):297–305

    Article  CAS  PubMed  Google Scholar 

  • Dimauro I, Mercatelli N, Caporossi D (2016) Exercise-induced ROS in heat shock proteins response. Free Radic Biol Med 98:46–55

    Article  CAS  PubMed  Google Scholar 

  • Drabovich AP, Jarvi K, Diamandis EP (2011) Verification of male infertility biomarkers in seminal plasma by multiplex selected reaction monitoring assay. Mol Cell Proteomics 10(12):M110.004127

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Duijvesz D, Luider T, Bangma CH, Jenster G (2011) Exosomes as biomarker treasure chests for prostate cancer. Eur Urol 59(5):823–831

    Article  CAS  PubMed  Google Scholar 

  • Eddy EM, Toshimori K, O’Brien DA (2003) Fibrous sheath of mammalian spermatozoa. Microsc Res Tech 61(1):103–115

    Article  CAS  PubMed  Google Scholar 

  • Elzanaty S, Richthoff J, Malm J, Giwercman A (2002) The impact of epididymal and accessory sex gland function on sperm motility. Hum Reprod 17(11):2904–2911

    Article  CAS  PubMed  Google Scholar 

  • Elzanaty S, Malm J, Giwercman A (2005) Duration of sexual abstinence: epididymal and accessory sex gland secretions and their relationship to sperm motility. Hum Reprod 20(1):221–225

    Article  PubMed  Google Scholar 

  • Erata GÖ, Toker NK, Durlanık Ö, Kadıoğlu A, Aktan G, Toker GA (2008) The role of heat shock protein 70 (Hsp 70) in male infertility: is it a line of defense against sperm DNA fragmentation? Fertil Steril 90(2):322–327

    Article  CAS  PubMed  Google Scholar 

  • Evans JP, Kopf G (1998) Molecular mechanisms of sperm–egg interactions and egg activation. Andrologia 30(4–5):297–307

    CAS  PubMed  Google Scholar 

  • Fourie M, Du Toit D, Bornman M, Van der Merwe M-P, Du Plessis D (1991) α-Glucosidase, sperm ATP concentrations, and epididymal function. Arch Androl 26(3):139–141

    Article  CAS  PubMed  Google Scholar 

  • Fraser LR, Harrison R, Herod JE (1990) Characterization of a decapacitation factor associated with epididymal mouse spermatozoa. Reproduction 89(1):135–148

    Article  CAS  Google Scholar 

  • Gaudet P, Livstone MS, Lewis SE, Thomas PD (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief Bioinform 12(5):449–462

    Article  PubMed  PubMed Central  Google Scholar 

  • Goodson SG, Qiu Y, Sutton KA, Xie G, Jia W, O’Brien DA (2012) Metabolic substrates exhibit differential effects on functional parameters of mouse sperm capacitation. Biol Reprod 87(3):75, 71-15

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Gosálvez J, González-Martínez M, López-Fernández C, Fernández JL, Sánchez-Martín P (2011) Shorter abstinence decreases sperm deoxyribonucleic acid fragmentation in ejaculate. Fertil Steril 96(5):1083–1086

    Article  PubMed  Google Scholar 

  • Goss DM, Ayad BM, van der Horst G, Skosana B, du Plessis SS (2019) Improved sperm motility after 4 h of ejaculatory abstinence: role of accessory sex gland secretions. Reprod Fertil Dev 31(5):1009–1016

    Article  CAS  PubMed  Google Scholar 

  • Graham J (1994) Effect of seminal plasma on the motility of epididymal and ejaculated spermatozoa of the ram and bull during the cryopreservation process. Theriogenology 41(5):1151–1162

    Article  CAS  PubMed  Google Scholar 

  • Guerin JF, Ali HB, Cottinet D, Rollet J (1990) Seminal alpha‐glucosidase activity as a marker of epididymal pathology in nonazoospermic men consulting for infertility. J Androl 11(3):240–245

    CAS  PubMed  Google Scholar 

  • Hamada A, Sharma R, du Plessis SS, Willard B, Yadav SP, Sabanegh E, Agarwal A (2013) Two-dimensional differential in-gel electrophoresis-based proteomics of male gametes in relation to oxidative stress. Fertil Steril 99(5):1216-1226.e1212

    Article  CAS  Google Scholar 

  • Heshmat SM, Mullen JB, Jarvi KA, Soosaipillai A, Diamandis EP, Hamilton RJ, Lo KC (2008) Seminal plasma lipocalin-type prostaglandin D synthase: a potential new marker for the diagnosis of obstructive azoospermia. J Urol 179(3):1077–1080

    Article  CAS  PubMed  Google Scholar 

  • Huang S, Kuo Y, Lee W, Tsou H, Lee Y, Chang H, Wu J, Yang P (1999) Substantial decrease of heat-shock protein 90 precedes the decline of sperm motility during cooling of boar spermatozoa. Theriogenology 51(5):1007–1016

    Article  CAS  PubMed  Google Scholar 

  • Huang S, Kuo Y, Tsou H, Lee Y, King Y, Huang H, Yang P, Lee W (2000) The decline of porcine sperm motility by geldanamycin, a specific inhibitor of heat-shock protein 90 (HSP90). Theriogenology 53(5):1177–1184

    Article  CAS  PubMed  Google Scholar 

  • Intasqui P, Camargo M, Del Giudice PT, Spaine DM, Carvalho VM, Cardozo KH, Zylbersztejn DS, Bertolla RP (2013) Sperm nuclear DNA fragmentation rate is associated with differential protein expression and enriched functions in human seminal plasma. BJU Int 112(6):835–843

    Article  CAS  PubMed  Google Scholar 

  • Intasqui P, Camargo M, Antoniassi MP, Cedenho AP, Carvalho VM, Cardozo KHM, Zylbersztejn DS, Bertolla RP (2016) Association between the seminal plasma proteome and sperm functional traits. Fertil Steril 105(3):617–628

    Article  CAS  PubMed  Google Scholar 

  • Iwasaki A, Gagnon C (1992) Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 57(2):409–416

    Article  CAS  PubMed  Google Scholar 

  • Jansen S, Ekhlasi-Hundrieser M, Töpfer-Petersen E (2001) Sperm adhesion molecules: structure and function. Cells Tissues Organs 168(1–2):82–92

    Article  CAS  PubMed  Google Scholar 

  • Kavanagh J (1994) Isocitric and citric acid in human prostatic and seminal fluid: implications for prostatic metabolism and secretion. Prostate 24(3):139–142

    Article  CAS  PubMed  Google Scholar 

  • Kessopoulou E, Tomlinson M, Barratt C, Bolton A, Cooke I (1992) Origin of reactive oxygen species in human semen: spermatozoa or leucocytes? Reproduction 94(2):463–470

    Article  CAS  Google Scholar 

  • Krause W, Bohring C (1999) Why do we determine α‐glucosidase activity in human semen during infertility work‐up?—Krause—1999—Andrologia—Wiley Online Library. Andrologia 31(5):289–294

    Article  CAS  PubMed  Google Scholar 

  • Lehavi O, Botchan A, Paz G, Yogev L, Kleiman S, Yavetz H, Hauser R (2014) Twenty‐four hours abstinence and the quality of sperm parameters. Andrologia 46(6):692–697

    Article  CAS  PubMed  Google Scholar 

  • Levitas E, Lunenfeld E, Weiss N, Friger M, Har-Vardi I, Koifman A, Potashnik G (2005) Relationship between the duration of sexual abstinence and semen quality: analysis of 9,489 semen samples. Fertil Steril 83(6):1680–1686

    Article  PubMed  Google Scholar 

  • Li D, Wang T, Wang X (2018) Characterization of sperm proteome and reproductive outcomes after reduced male abstinence in IVF treatment. Fertil Steril 110(4):e296

    Article  Google Scholar 

  • Lu Q, Sun QY, Breitbart H, Chen DY (1999) Expression and phosphorylation of mitogen-activated protein kinases during spermatogenesis and epididymal sperm maturation in mice. Archiv Androl 43(1):55–66

    Article  CAS  Google Scholar 

  • Mann T (1946) Studies on the metabolism of semen: 3. Fructose as a normal constituent of seminal plasma. Site of formation and function of fructose in semen. Biochem J 40(4):481

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mann T (1967) Sperm metabolism. In: Fertilization. Elsevier, Amsterdam, pp 99–116

    Chapter  Google Scholar 

  • Mann T, Lutwak-Mann C (1981) Male reproductive function and the composition of semen: general considerations. In: Male reproductive function and semen. Springer, Berlin, pp 1–37

    Chapter  Google Scholar 

  • Marshburn PB, Alanis M, Matthews ML, Usadi R, Papadakis MH, Kullstam S, Hurst BS (2010) A short period of ejaculatory abstinence before intrauterine insemination is associated with higher pregnancy rates. Fertil Steril 93(1):286–288

    Article  PubMed  Google Scholar 

  • Marshburn PB, Giddings A, Causby S, Matthews ML, Usadi RS, Steuerwald N, Hurst BS (2014) Influence of ejaculatory abstinence on seminal total antioxidant capacity and sperm membrane lipid peroxidation. Fertil Steril 102(3):705–710

    Article  CAS  PubMed  Google Scholar 

  • Martínez‐Heredia JEJMBJL, Oliva R (2006) Proteomic identification of human sperm proteins. Proteomics 6:15

    Article  CAS  Google Scholar 

  • Mayorga-Torres BJ, Camargo M, Agarwal A, du Plessis SS, Cadavid AP, Cardona Maya WD (2015) Influence of ejaculation frequency on seminal parameters. Reprod Biol Endocrinol 13:47

    Article  PubMed  PubMed Central  Google Scholar 

  • Medrano A, Fernández‐Novell JM, Ramió L, Alvarez J, Goldberg E, Montserrat Rivera M, Guinovart JJ, Rigau T, Rodríguez‐Gil JE (2006) Utilization of citrate and lactate through a lactate dehydrogenase and ATP‐regulated pathway in boar spermatozoa. Molecul Reprod Develop Incorporat Gamete Res 73(3):369–378

    Article  CAS  Google Scholar 

  • Miki K, Qu W, Goulding EH, Willis WD, Bunch DO, Strader LF, Perreault SD, Eddy EM, O’Brien DA (2004) Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility. Proc Natl Acad Sci 101(47):16501–16506

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Milardi D, Grande G, Vincenzoni F, Messana I, Pontecorvi A, De Marinis L, Castagnola M, Marana R (2012) Proteomic approach in the identification of fertility pattern in seminal plasma of fertile men. Fertil Steril 97(1):67–73.e61

    Article  CAS  Google Scholar 

  • Mukai C, Okuno M (2019) Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement. Biol Reprod 71(2):540–547

    Article  CAS  Google Scholar 

  • Mukherjee SLPCELRVWCHCLBC, Hooper LV (2009) Regulation of C-type lectin antimicrobial activity by a flexible N-terminal prosegment. J Biol Chem 284(8):4881–4888

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mustafi SB, Chakraborty PK, Dey RS, Raha S (2009) Heat stress upregulates chaperone heat shock protein 70 and antioxidant manganese superoxide dismutase through reactive oxygen species (ROS), p38MAPK, and Akt. Cell Stress Chaperones 14(6):579

    Article  CAS  Google Scholar 

  • NagDas SK, Winfrey VP, Olson GE (2002) Identification of ras and its downstream signalling elements and their potential role in hamster sperm motility. Biol Reprod 67(4):1058–1066

    Article  CAS  PubMed  Google Scholar 

  • Naz RK, Ahmad K, Kaplan P (1992) Expression and function of ras proto-oncogene proteins in human sperm cells. J Cell Sci 102(3):487–494

    Article  CAS  PubMed  Google Scholar 

  • Oliva R, de Mateo S, Estanyol JM (2009) Sperm cell proteomics. Proteomics 9(4):1004–1017

    Article  CAS  PubMed  Google Scholar 

  • Patel S, Skandhan K, Mehta Y (1988) Seminal plasma fructose and glucose in normal and pathological conditions. Acta Eur Fertil 19(6):329–332

    CAS  PubMed  Google Scholar 

  • Pilch B, Mann M (2006) Large-scale and high-confidence proteomic analysis of human seminal plasma. Genome Biol 7(5):R40

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Poliakov A, Spilman M, Dokland T, Amling CL, Mobley JA (2009) Structural heterogeneity and protein composition of exosome‐like vesicles (prostasomes) in human semen. Prostate 69(2):159–167

    Article  PubMed  Google Scholar 

  • Primakoff P, Myles DG (2002) Penetration, adhesion, and fusion in mammalian sperm-egg interaction. Science 296(5576):2183–2185

    Article  CAS  PubMed  Google Scholar 

  • Raziel A, Friedler S, Schachter M, Kaufman S, Omanski A, Soffer Y, Ron-El R (2001) Influence of a short or long abstinence period on semen parameters in the ejaculate of patients with nonobstructive azoospermia. Fertil Steril 76(3):485–490

    Article  CAS  PubMed  Google Scholar 

  • Rees JM, Ford W, Hull M (1990) Effect of caffeine and of pentoxifylline on the motility and metabolism of human spermatozoa. Reproduction 90(1):147–156

    Article  CAS  Google Scholar 

  • Rodriguez‐Gil J (2006) Mammalian sperm energy resources management and survival during conservation in refrigeration. Reprod Domest Anim 41:11–20

    Article  PubMed  Google Scholar 

  • Rotem R, Paz GF, Homonnai ZT, Kalina M, Naor Z (1990) Protein kinase C is present in human sperm: possible role in flagellar motility. Proc Natl Acad Sci 87(18):7305–7308

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Said L, Galeraud‐Denis I, Carreau S, Saâd A (2009) Relationship between semen quality and seminal plasma components: alpha‐glucosidase, fructose and citrate in infertile men compared with a normospermic population of Tunisian men—Said—2009—Andrologia—Wiley Online Library. Andrologia 41(3):150–156

    Article  CAS  PubMed  Google Scholar 

  • Scarselli F, Cursio E, Muzzì S, Casciani V, Ruberti A, Gatti S, Greco P, Varricchio MT, Minasi MG, Greco E (2019) How 1 h of abstinence improves sperm quality and increases embryo euploidy rate after PGT-A: a study on 106 sibling biopsied blastocysts. J Assist Reprod Genet 36(8):1591–1597

    Article  PubMed  PubMed Central  Google Scholar 

  • Schoenfeld CYARDDL, Numeroff M (1979) Prolactin, fructose, and zinc levels found in human seminal plasma. Fertil Steril 32(2):206–208

    Article  CAS  PubMed  Google Scholar 

  • Shacoski JC (2012) Heat shock proteins as markers for cellular stress in mammalian sperm. University of California, Davis

    Google Scholar 

  • Sharma R, Agarwal A, Mohanty G, Du Plessis SS, Gopalan B, Willard B, Yadav SP, Sabanegh E (2013a) Proteomic analysis of seminal fluid from men exhibiting oxidative stress. Reprod Biol Endocrinol 11:85

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Sharma R, Agarwal A, Mohanty G, Hamada AJ, Gopalan B, Willard B, Yadav S, du Plessis S (2013b) Proteomic analysis of human spermatozoa proteins with oxidative stress. Reprod Biol Endocrinol 11:48

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shen Z, Shi B, Wang T, Jiao J, Shang X, Wu Q, Zhou Y, Cao T, Du Q, Wang X, Li D (2019) Characterization of the sperm proteome and reproductive outcomes with in vitro, fertilization after a reduction in male ejaculatory abstinence period. Mol Cell Proteomics 18(1):S109–S117

    Article  PubMed  Google Scholar 

  • Simpson RJ, Lim JWE, Moritz RL, Mathivanan S (2009) Exosomes: proteomic insights and diagnostic potential. Exp Rev Proteom 6(3):267–283

    Article  CAS  Google Scholar 

  • Storey BT (2004) Mammalian sperm metabolism: oxygen and sugar, friend and foe. Int J Developm Biol 52:427–437

    Article  CAS  Google Scholar 

  • Storey BT, Kayne FJ (1975) Energy metabolism of spermatozoa. V. The Embden-Myerhof pathway of glycolysis: activities of...—Abstract—Europe PMC. Fertil Steril 26(12):1257–1265

    Article  CAS  PubMed  Google Scholar 

  • Travis AJFJARNAVPEGGLKGS, Moss SB (1998) Targeting of a germ cell-specific type 1 hexokinase lacking a porin-binding domain to the mitochondria as well as to the head and fibrous sheath of murine spermatozoa. Mol Biol Cell 9(2):263–276

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Turner RM (2003) Tales from the tail: what do we really know about sperm motility? J Androl 24(6):790–803

    Article  PubMed  Google Scholar 

  • Turpeenniemi-Hujanen TMPU, Kivirikko KI (1980) Isolation of lysyl hydroxylase, an enzyme of collagen synthesis, from chick embryos as a homogeneous protein. Biochem J 189(2):247–253

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Viljoen MHBMSVDMMP, Du Plessis DJ (1990) Alpha‐glucosidase activity and sperm motility. Andrologia 22(3):205–208

    Article  CAS  PubMed  Google Scholar 

  • Visconti PE (2012) Sperm bioenergetics in a nutshell. Biol Reprod 87(3):72

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang J, Zhang HR, Shi HJ, Ma D, Zhao HX, Lin B, Li RS (2009) Proteomic analysis of seminal plasma from asthenozoospermia patients reveals proteins that affect oxidative stress responses and semen quality. Asian J Androl 11(4):484–491

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Westhoff D, Kamp G (1997) Glyceraldehyde 3-phosphate dehydrogenase is bound to the fibrous sheath of mammalian spermatozoa. J Cell Sci 110(15):1821–1829

    Article  CAS  PubMed  Google Scholar 

  • WHO (2010) WHO laboratory manual for the examination and processing of human semen, 5th edn. World Health Organization, Geneva

    Google Scholar 

  • Williams AC, Ford WCL (2001) The role of glucose in supporting motility and capacitation in human spermatozoa. J Androl 22(4):680–695

    CAS  PubMed  Google Scholar 

  • Yi Y-J, Manandhar G, Sutovsky M, Li R, Jonáková V, Oko R, Sutovsky P (2007) Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization. Biol Reprod 77(5):780–793

    Article  CAS  PubMed  Google Scholar 

  • Zhang M, Wang D, Geng Z, Li P, Sun Z, Xu W (2018) Effect of heat shock protein 90 against ROS-induced phospholipid oxidation. Food Chem 240:642–647

    Article  CAS  PubMed  Google Scholar 

  • Zylbersztejn DSACDGPTSDMBLSGH, Fraietta R (2013) Proteomic analysis of seminal plasma in adolescents with and without varicocele. Fertil Steril 99(1):92–98

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Medical Physiology, Stellenbosch University, Tygerberg, South Africa

    Dale M. Goss, Bashir Ayad, Suzél M. Hattingh, Gerhard van der Horst & Stefan S. du Plessis

  2. Department of Biochemistry and LCMS-Central Analytical Facility, Stellenbosch University, Tygerberg, South Africa

    Maré Vlok

  3. Department of Basic Medical Sciences, College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, UAE

    Stefan S. du Plessis

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  1. Dale M. Goss
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  2. Bashir Ayad
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Correspondence to Stefan S. du Plessis .

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  1. Andrology Laboratory, ANOVA Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden

    Lars Björndahl

  2. Andrology Laboratory, ANOVA Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden

    John Flanagan

  3. Andrology Laboratory, ANOVA Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden

    Rebecka Holmberg

  4. Andrology Laboratory, ANOVA Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden

    Ulrik Kvist

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Goss, D.M., Ayad, B., Vlok, M., Hattingh, S.M., van der Horst, G., du Plessis, S.S. (2021). Proteomics and Biomarker Identification in Improved Sperm Motility Parameters After 4 h of Ejaculatory Abstinence. In: Björndahl, L., Flanagan, J., Holmberg, R., Kvist, U. (eds) XIIIth International Symposium on Spermatology. Springer, Cham. https://doi.org/10.1007/978-3-030-66292-9_48

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