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An Introduction to Reproducibility in the Context of Animal Research

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Experimental Design and Reproducibility in Preclinical Animal Studies

Part of the book series: Laboratory Animal Science and Medicine ((LASM,volume 1))

Abstract

This book’s aim is to improve animal research, so they are used only when needed, provide reliable information and are not wasted. This is not a book about fraud in science.

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References

  1. Russell WMS, Burch RL. The principles of humane experimental technique. London: Methuen & Co Limited; 1959.

    Google Scholar 

  2. Ramón y Cajal S. Estructura de los centros nerviosos de las aves. Revista trimestral de Histología normal y patológica. 1888;1:1–10.

    Google Scholar 

  3. Ramón y Cajal S. Morfología y conexiones de los elementos de la retina de las aves. Revista trimestral de Histología normal y patológica. 1888;1:11–6.

    Google Scholar 

  4. Ramón y Cajal S. Terminaciones nerviosas en los husos musculares de la rana. Revista trimestral de Histología normal y patológica. 1888;1:16–8.

    Google Scholar 

  5. Ramón y Cajal S. Sobre las fibras nerviosas de la capa molecular del cerebelo. Revista trimestral de Histología normal y patológica. 1888;1:33–42.

    Google Scholar 

  6. Ramón y Cajal S. Extructura de la retina de las aves. Revista trimestral de Histología normal y patológica. 1888;1:42–9.

    Google Scholar 

  7. Ramón y Cajal S. Nota sobre la estructura de los tubos nerviosos del lóbulo cerebral eléctrico del torpedo. Revista trimestral de Histología normal y patológica. 1888;1:49–57.

    Google Scholar 

  8. Golgi C. The neuron doctrine – theory and facts. In: Lindsten J, editor. Nobel lectures, physiology or medicine, 1901–1921. London: World Scientific Publishing Co; 1999. p. 189–217.

    Google Scholar 

  9. Franklin RE, Gosling RG. Evidence for 2-chain helix in crystalline structure of sodium deoxyribonucleate. Nature. 1953;172:156–7. https://doi.org/10.1038/172156a0.

    Article  CAS  PubMed  Google Scholar 

  10. Franklin RE, Gosling RG. Molecular configuration in sodium thymonucleate. Nature. 1953;171:740–1. https://doi.org/10.1038/171740a0.

    Article  CAS  PubMed  Google Scholar 

  11. Wilkins MH, Stokes AR, Wilson HR. Molecular structure of deoxypentose nucleic acids. Nature. 1953;171:738–40. https://doi.org/10.1038/171738a0.

    Article  CAS  PubMed  Google Scholar 

  12. Watson JD, Crick FH. Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature. 1953;171:737–8. https://doi.org/10.1038/171737a0.

    Article  CAS  Google Scholar 

  13. Pauling L, Corey RBA. Proposed structure for the nucleic acids. Proc Natl Acad Sci U S A. 1953;39:84–97. https://doi.org/10.1073/pnas.39.2.84.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Goodman SN, Fanelli D, Ioannidis JPA. What does research reproducibility mean? Sci Trans Med. 2016;8:341ps312–341ps312. https://doi.org/10.1126/scitranslmed.aaf5027.

    Article  Google Scholar 

  15. Casadevall A, Fang FC. Reproducible science. Infect Immun. 2010;78:4972–5. https://doi.org/10.1128/IAI.00908-10.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Voelkl B, Vogt L, Sena ES, et al. Reproducibility of preclinical animal research improves with heterogeneity of study samples. PLoS Biol. 2018;16:e2003693–e2003693. https://doi.org/10.1371/journal.pbio.2003693.

    Article  CAS  Google Scholar 

  17. Ward JM, Schofield PN, Sundberg JP. Reproducibility of histopathological findings in experimental pathology of the mouse: a sorry tail. Lab Anim. 2017;46:146–51. https://doi.org/10.1038/laban.1214.

    Article  Google Scholar 

  18. Brinster RL, Chen HY, Trumbauer M, et al. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell. 1981;27:223–31. https://doi.org/10.1016/0092-8674(81)90376-7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Gordon JW, Ruddle FH. Integration and stable germ line transmission of genes injected into mouse pronuclei. Science. 1981;214:1244–6. https://doi.org/10.1126/science.6272397.

    Article  CAS  PubMed  Google Scholar 

  20. Costantini F, Lacy E. Introduction of a rabbit beta-globin gene into the mouse germ line. Nature. 1981;294:92–4. https://doi.org/10.1038/294092a0.

    Article  CAS  PubMed  Google Scholar 

  21. Dobrowolski P, Fischer M, Naumann R. Novel insights into the genetic background of genetically modified mice. Transgenic Res. 2018;27:265–75. https://doi.org/10.1007/s11248-018-0073-2.

    Article  CAS  PubMed  Google Scholar 

  22. Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014;346:1258096. https://doi.org/10.1126/science.1258096.

    Article  CAS  PubMed  Google Scholar 

  23. Tuttle AH, Philip VM, Chesler EJ, et al. Comparing phenotypic variation between inbred and outbred mice. Nat Methods. 2018;15:994–6. https://doi.org/10.1038/s41592-018-0224-7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Lusis AJ, Yu J, Wang SS. The problem of passenger genes in transgenic mice. Arterioscler Thromb Vasc Biol. 2007;27:2100–3. https://doi.org/10.1161/ATVBAHA.107.147918.

    Article  CAS  PubMed  Google Scholar 

  25. Richter SH, Garner JP, Würbel H. Environmental standardization: cure or cause of poor reproducibility in animal experiments? Nat Methods. 2009;6:257–61. https://doi.org/10.1038/nmeth.1312.

    Article  CAS  PubMed  Google Scholar 

  26. Richter SH, Garner JP, Auer C, et al. Systematic variation improves reproducibility of animal experiments. Nat Methods. 2010;7:167–8. https://doi.org/10.1038/nmeth0310-167.

    Article  CAS  PubMed  Google Scholar 

  27. Richter SH, Garner JP, Zipser B, et al. Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study. PLoS One. 2011;6:e16461–e16461. https://doi.org/10.1371/journal.pone.0016461.

    Article  Google Scholar 

  28. European C. Commision recommendation of 18 June 2007 on guidelines for the accommodation and care of animals used for experimental and other scientific purposes. Off J Eur Union. 2007;2003

    Google Scholar 

  29. Mohamed AS, Fahmy SR, Soliman AM, et al. Effects of 3 rodent beddings on biochemical measures in rats and mice. J Am Assoc Lab Anim Sci. 2018;57:443–6. https://doi.org/10.30802/AALAS-JAALAS-18-000023.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Pain terms: a list with definitions and notes on usage. Recommended by the IASP Subcommittee on Taxonomy. Pain. 1979;6:249.

    Google Scholar 

  31. Raja SN, Carr DB, Cohen M, et al. The revised International Association for the Study of Pain definition of pain: concepts, challenges, and compromises. Pain. 2020; https://doi.org/10.1097/j.pain.0000000000001939.

  32. Greek R, Menache A. Systematic reviews of animal models: methodology versus epistemology. Int J Med Sci. 2013;10:206–21. Review. https://doi.org/10.7150/ijms.5529.

    Article  PubMed  PubMed Central  Google Scholar 

  33. Kilkenny C, Browne WJ, Cuthill IC, et al. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol. 2010;8:e1000412. https://doi.org/10.1371/journal.pbio.1000412.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Leung V, Rousseau-Blass F, Beauchamp G, et al. ARRIVE has not ARRIVEd: Support for the ARRIVE (Animal Research: Reporting of in vivo Experiments) guidelines does not improve the reporting quality of papers in animal welfare, analgesia or anesthesia. PLOS One. 2018;13:e0197882. https://doi.org/10.1371/journal.pone.0197882.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Percie du Sert N, Hurst V, Ahluwalia A, et al. The ARRIVE guidelines 2.0: updated guidelines for reporting animal research. PLoS Biol. 2020;18:e3000410. https://doi.org/10.1371/journal.pbio.3000410.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Smith AJ, Clutton RE, Lilley E, et al. Improving animal research: PREPARE before you ARRIVE. BMJ. 2018;360:k760. https://doi.org/10.1136/bmj.k760.

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Comparative Medicine, Trinity College Dublin, TheUniversity of Dublin, Dublin, Ireland

    José M. Sánchez-Morgado

  2. Department of Clinical Medicine, University of Bergen, Bergen, Norway

    Aurora Brønstad

Authors
  1. José M. Sánchez-Morgado
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  2. Aurora Brønstad
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Corresponding author

Correspondence to José M. Sánchez-Morgado .

Editor information

Editors and Affiliations

  1. Comparative Medicine, Trinity College Dublin Comparative Medicine, Dublin, Ireland

    José M. Sánchez Morgado

  2. University of Bergen, Laboratory Animal Science University of Bergen, Bergen, Norway

    Aurora Brønstad

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Sánchez-Morgado, J.M., Brønstad, A. (2021). An Introduction to Reproducibility in the Context of Animal Research. In: Sánchez Morgado, J.M., Brønstad, A. (eds) Experimental Design and Reproducibility in Preclinical Animal Studies . Laboratory Animal Science and Medicine, vol 1. Springer, Cham. https://doi.org/10.1007/978-3-030-66147-2_1

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