Abstract
In this paper, we propose a new method to obtain the improvement of lateral axial resolution of confocal fluorescence microscopy. In this method, we employ two different beams to illuminate the sample: (1) the Gaussian beam; (2) the donut beam. Two different images are produced from these two illumination beams. A higher resolution image is generated by a multi-relationship between these two image. A set of simulation and experimental results are employed to compare the effectiveness of proposed method with the traditional confocal fluorescence microscopy. These results demonstrated that our method can be employed to achieve the resolution-enhancement of confocal fluorescence microscopy.
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Acknowledgment
This work is supported by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under Grant number (103.03-2018.08).
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Hoang, X., Le, V., Pham, M. (2020). Resolution-Improvement of Confocal Fluorescence Microscopy via Two Different Point Spread Functions. In: Vo, NS., Hoang, VP. (eds) Industrial Networks and Intelligent Systems. INISCOM 2020. Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering, vol 334. Springer, Cham. https://doi.org/10.1007/978-3-030-63083-6_6
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DOI: https://doi.org/10.1007/978-3-030-63083-6_6
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