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In Vitro Assays for Nanoparticle—Cancer Cell Interaction Studies

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Bio-Nanomedicine for Cancer Therapy

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1295))

Abstract

Nanotechnology is a rapid-growing field with an extreme potential to revolutionize cancer treatments. However, despite the rapid advances, the clinical translation is still scarce. One of the main hurdles contributing for this setback is the lack of reliable in vitro models for preclinical testing capable of predicting the outcomes in an in vivo setting. In fact, the use of 2D monolayers, considered the gold-standard in vitro technique, leads to the creation of misleading data that might not be completely observed in in vivo or clinical setting. Thus, there is the need to use more complex models capable of better mimicking the tumor microenvironment. For that purpose, the development and use of multicellular tumor spheroids, three-dimensional (3D) cell cultures which recapitulate numerous aspects of the tumors, represents an advantageous approach to test the developed anticancer therapies. In this chapter, we identify and discuss the advantages of the use of these 3D cellular models compared to the 2D models and how they can be utilized to study nanoparticle-cancer cell interaction in a more reliable way to predict the treatment outcome in vivo.

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Acknowledgments

T. Bauleth-Ramos acknowledges financial support from Fundação para a Ciência e a Tecnologia (grant no. SFRH/BD/110859/2015). This work was financed by the project NORTE-01-0145-FEDER-000012 by Norte Portugal Regional Operational Programme (NORTE 2020), and COMPETE 2020 – Operacional Programme for Competitiveness and Internationalisation (POCI), under the PORTUGAL 2020 Partnership Agreement, through the (FEDER) Fundo Europeu de Desenvolvimento Regional and by Portuguese funds through (FCT) Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior in the framework of the project “Institute for Research and Innovation in Health Sciences” UID/BIM/04293/2019.

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Authors and Affiliations

  1. i3S – Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal

    Tomás Bauleth-Ramos & Bruno Sarmento

  2. INEB – Instituto de Engenharia Biomédica, University of Porto, Porto, Portugal

    Tomás Bauleth-Ramos & Bruno Sarmento

  3. ICBAS, Instituto Ciências Biomédicas Abel Salazar, University of Porto, Porto, Portugal

    Tomás Bauleth-Ramos

  4. Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland

    Tomás Bauleth-Ramos

  5. CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde & Instituto Universitário de Ciências da Saúde, Gandra, Portugal

    Bruno Sarmento

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  1. Tomás Bauleth-Ramos
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Correspondence to Bruno Sarmento .

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  1. Faculty of Pharmacy, University of Helsinki, Helsinki, Finland

    Flavia Fontana

  2. Faculty of Pharmacy, University of Helsinki, Helsinki, Finland

    Hélder A. Santos

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Bauleth-Ramos, T., Sarmento, B. (2021). In Vitro Assays for Nanoparticle—Cancer Cell Interaction Studies. In: Fontana, F., Santos, H.A. (eds) Bio-Nanomedicine for Cancer Therapy. Advances in Experimental Medicine and Biology, vol 1295. Springer, Cham. https://doi.org/10.1007/978-3-030-58174-9_10

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