Abstract
Many farmers use antibiotics in livestock and poultry production. The aim of this study was to evaluate the ability of lettuce and radish to absorb these antibiotics from two growth media (soil amended with 5% manure and soil without manure) both spiked with antibiotics (0, 2.5, 5, and 10 mg/kg). A factorial completely randomized pot experiment was conducted in the greenhouse of the American University of Beirut, Lebanon. The antibiotic analysis was done by ELISA. The results showed that gentamicin accumulated in lettuce roots (12.7 ng/g) and leaves (17.7 ng/g) at approximately equimolar ratio (1:1 ratio) whereas in radish it accumulated in the leaves (31.5 ng/g) at a higher concentration than in the roots (16.4 ng/g) (2:1 ratio, leaves:roots). Tylosin was absorbed only at the highest concentration treatment (10 mg/kg) by lettuce roots (11.2 ng/g) with a limited translocation to the leaves (3.58 ng/g), whereas in radish a higher absorption and accumulation of tylosin was observed in both the roots (56.6 ng/g) and leaves (62.9 ng/g). Oxytetracycline was not absorbed by lettuce but it accumulated in radish at low levels (1.83–3.98 ng/g) in the roots and 4.85–6.69 ng/g in the leaves. The addition of manure to the soil enhanced the uptake of gentamicin and tylosin but not oxytetracycline. The results indicated also that increasing the concentrations of antibiotics in the growing media did not always lead to a significant increase in the concentrations of antibiotics in plant tissues.
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Acknowledgments
This study was supported by a research grant from the National Council for Scientific Research (CNRS), Lebanon. The technical advice of Dr. Hussam Shaib and the support and help of Ms. Hana Soboh in the antibiotics analysis are greatly appreciated.
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Youssef, S.A., Bashour, I.I., Abou Jawdeh, Y.A., Farran, M.T., Farajalla, N. (2020). Uptake of Gentamicin, Oxytetracycline, and Tylosin by Lettuce and Radish Plants. In: Hashmi, M. (eds) Antibiotics and Antimicrobial Resistance Genes. Emerging Contaminants and Associated Treatment Technologies. Springer, Cham. https://doi.org/10.1007/978-3-030-40422-2_9
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