Abstract
Biocomposite material based on hydroxyapatite (HA) and chitosan (CS) in form of beads was synthesized using microwave irradiation (MW) of different power and investigated by methods of XRD and HPLC. MW reduces the time of HA formation to 20 min without affecting its structure. Liquid absorption and liquid retention for the synthesized at maximum MW power HA-CS-MW800 sample is 27%, while for the control HA-CS 5%. HPLC showed significant differences in the release kinetics of the hydrophilic Chlorhexidine (CHX) and hydrophobic benzocaine (BZ) and proved the composite ability to prolonged drug release to 336 h for CHX and 384 h for BZ. The HA-CS-MW800 demonstrates the highest release rate for CHX and the lowest one for BZ. From HA-CS-MW800 sample in the first 2 h is released the smallest amount of BZ (about 32%), compared to 45% for others. The effect of MW contributes to the specific location of binding sites in composite and access of drug molecules to these sites.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Melo, M.A.S., Cheng, L., Weir, M.D., Hsia, R.-C., Rodrigues, L.K.A., Xu, H.H.K.: Novel dental adhesive containing antibacterial agents and calcium phosphate nanoparticles. J. Biomed. Mater. Res. B Appl. Biomater. 101B(4), 620–629 (2012)
Sukhodub, L.F., Dyadyura, K.: Design and fabrication of polymer-ceramic nanocomposites materials for bone tissue engineering. J. Nano- Electron. Phys. 10(6), 06003 (2018)
Jain, A., Duvvuri, L.S., Farah, S., Beyth, N., Domb, A.J., Khan, W.: Antimicrobial polymers. Adv. Healthc. Mater. 3(12), 1969–1985 (2014)
Casettari, L., Illum, L.: Chitosan in nasal delivery systems for therapeutic drugs. J. Control. Release 190, 189–200 (2014). https://doi.org/10.1016/j.jconrel.2014.05.003
Katarina, R.K., Takayanagi, T., Oshima, M., Motomizu, S.: Synthesis of a chitosan-based chelating resin and its application to the selective concentration and ultratrace determination of silver in environmental water samples. Anal. Chim. Acta 558(1–2), 246–253 (2006). https://doi.org/10.1016/j.aca.2005.11.010
Park, J., Kim, Y., Choi, S., Koh, H., Lee, S.-H., Kim, J.-M., Chung, J.: Drosophila porin/VDAC affects mitochondrial morphology. PLoS ONE 5(10), e13151 (2010). https://doi.org/10.1371/journal.pone.0013151
Beenken, K.E., Mrak, L.N., Zielinska, A.K., Atwood, D.N., Loughran, A.J., Griffin, L.M., Smeltzer, M.S.: Impact of the functional status of saeRS on in vivo phenotypes of Staphylococcus aureus sarA mutants. Mol. Microbiol. 92(6), 1299–1312 (2014). https://doi.org/10.1111/mmi.12629
Harris, M., Alexander, C., Wells, C.M., Bumgardner, J.D., Carpenter, D.P., Jennings, J.A.: Chitosan for the delivery of antibiotics. Chitosan Based Biomater. 2, 147–173 (2017). https://doi.org/10.1016/b978-0-08-100228-5.00006-7
Sukhodub, L.: Metal ions doped chitosan nanoparticles. J. Nano- Electron. Phys. 6 (2014)
Neiders, M.E., Weiss, L.: The effects of chlorhexidine on cell detachment in vitro. Arch. Oral Biol. 17(6), 961–967 (1972). https://doi.org/10.1016/0003-9969(72)90119-7
Han, Y., Zeng, Q., Li, H., Chang, J.: The calcium silicate/alginate composite: preparation and evaluation of its behavior as bioactive injectable hydrogels. Acta Biomater. 9(11), 9107–9117 (2013). https://doi.org/10.1016/j.actbio.2013.06.022
Venkatesan, J., Qian, Z.-J., Ryu, B., Ashok Kumar, N., Kim, S.-K.: Preparation and characterization of carbon nanotube-grafted-chitosan – natural hydroxyapatite composite for bone tissue engineering. Carbohydr. Polym. 83(2), 569–577 (2011). https://doi.org/10.1016/j.carbpol.2010.08.019
Klug, H.P., Alexander, L.E.: X-ray Diffraction Procedures for Polycrystalline and Amorphous Materials. Wiley, New York (1974)
Oliveira, P.M., Matos, B.N., Pereira, P.A.T., Gratieri, T., Faccioli, L.H., Cunha-Filho, M.S.S., Gelfuso, G.M.: Microparticles prepared with 50–190 kDa chitosan as promising non-toxic carriers for pulmonary delivery of isoniazid. Carbohydr. Polym. 174, 421–431 (2017). https://doi.org/10.1016/j.carbpol.2017.06.090
Conflict of Interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this paper
Cite this paper
Sukhodub, L.B., Kumeda, M.O., Sukhodub, L.F. (2020). Influence of MW Irradiation on the Hydroxyapatite/Chitosan Composite Structure and Drug Release Kinetics. In: Tiginyanu, I., Sontea, V., Railean, S. (eds) 4th International Conference on Nanotechnologies and Biomedical Engineering. ICNBME 2019. IFMBE Proceedings, vol 77. Springer, Cham. https://doi.org/10.1007/978-3-030-31866-6_64
Download citation
DOI: https://doi.org/10.1007/978-3-030-31866-6_64
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-31865-9
Online ISBN: 978-3-030-31866-6
eBook Packages: EngineeringEngineering (R0)