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Co-encapsulation of Beta Cells and Nanoparticles Containing GLP-1 Greatly Improves Insulin Secretion in Alginate-Based Bioartificial Pancreas

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XV Mediterranean Conference on Medical and Biological Engineering and Computing – MEDICON 2019 (MEDICON 2019)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 76))

Abstract

The bioartificial pancreas presents a promising methodology to treat diabetes. However, this approach is still associated with a high rate of graft failure caused by poorly functioning cells. The incretin effect of glucagon like peptide-1 (GLP-1) make this peptide an attractive therapeutic agent to improve the success of transplantation of encapsulated beta cells. In present work, we developed a novel model based on the co-encapsulation of beta cells with nanoparticles containing GLP-1 on alginate hydrogels, allowing its delivery and action in the specific target, the beta cells.

INS-1E beta cells were co-encapsulated with poly(lactide-co-glycolide) (PLGA) nanoparticles containing GLP-1 in alginate and were maintained in culture aiming to evaluate the performance of our drug-delivery system and the influence of GLP-1 in cellular performance.

PLGA nanoparticles showed an average size of 169.3 nm, a polydispersity index of 0.05 and an average zeta-potential of −24.3 mV. The average association efficiency of GLP-1 was 65.4% and the in vitro release test showed 71% of GLP-1 delivery after 7 days. The presence of nanoparticles led to increased cellular metabolic activity and 8-fold higher insulin levels. Our results reveal a promising approach to ensure the proper delivery and action of GLP-1 in encapsulated beta cells, ensuring its viability.

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Acknowledgements

The present work is a collaboration between IBILI and INEB, supported by grants UID/NEU/04539/2013, UID/BIM/04293/2013 and SFRH/BD/72964/2010.

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Authors and Affiliations

  1. Laboratory of Physiology, iCBR - Coimbra Institute for Clinical and Biomedical Research, Faculty of Medicine, University of Coimbra, Coimbra, Portugal

    Joana Crisóstomo & Raquel Seiça

  2. INEB - Instituto de Engenharia Biomédica, Porto, Portugal

    Francisca Araújo, Pedro Granja, Cristina Barrias & Bruno Sarmento

  3. i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, ‎Porto, Portugal

    Francisca Araújo, Pedro Granja, Cristina Barrias & Bruno Sarmento

  4. FEUP - Faculdade de Engenharia, Universidade do Porto, ‎Porto, Portugal

    Pedro Granja

  5. ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, ‎Porto, Portugal

    Pedro Granja & Cristina Barrias

Authors
  1. Joana Crisóstomo
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  2. Francisca Araújo
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  3. Pedro Granja
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  4. Cristina Barrias
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  5. Bruno Sarmento
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  6. Raquel Seiça
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Corresponding author

Correspondence to Joana Crisóstomo .

Editor information

Editors and Affiliations

  1. University of Coimbra, Coimbra, Portugal

    Jorge Henriques

  2. Universidade do Minho, Braga, Portugal

    Nuno Neves

  3. University of Coimbra, Coimbra, Portugal

    Paulo de Carvalho

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Crisóstomo, J., Araújo, F., Granja, P., Barrias, C., Sarmento, B., Seiça, R. (2020). Co-encapsulation of Beta Cells and Nanoparticles Containing GLP-1 Greatly Improves Insulin Secretion in Alginate-Based Bioartificial Pancreas. In: Henriques, J., Neves, N., de Carvalho, P. (eds) XV Mediterranean Conference on Medical and Biological Engineering and Computing – MEDICON 2019. MEDICON 2019. IFMBE Proceedings, vol 76. Springer, Cham. https://doi.org/10.1007/978-3-030-31635-8_147

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  • DOI: https://doi.org/10.1007/978-3-030-31635-8_147

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-31634-1

  • Online ISBN: 978-3-030-31635-8

  • eBook Packages: EngineeringEngineering (R0)

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