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Basic and Ancillary Techniques in Bone Pathology

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Tumors and Tumor-Like Lesions of Bone

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Abstract

In skeletal pathology diagnosis, most tumors and tumorlike conditions can be diagnosed with routine hematoxylin and eosin (H&E) stain, but sometimes this basic technique can be insufficient. Histochemical methods evaluate the state of the ossification process and intracellular activated enzymes (PAS, mucin stain, Congo red, von Kossa, etc.). Electron microscopy, flow cytometry, and histomorphometry have limited diagnostic uses. Immunohistochemical staining has been a helpful aid, especially in the diagnosis of round-cell tumors, metastatic disease, Langerhans cell histiocytosis, and vascular tumors. Karyotyping, molecular cytogenetic techniques such as FISH, and molecular techniques like blotting, PCR, and others have limited but effective application in the diagnosis of some specific bone tumors.

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Author information

Authors and Affiliations

  1. Department of Pathology, Kyung Hee University Hospital, Seoul, Korea

    Kiyong Na & Yong-Koo Park

Authors
  1. Kiyong Na
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  2. Yong-Koo Park
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Editor information

Editors and Affiliations

  1. Department of Pathology, University of Buenos Aires, Buenos Aires, Argentina

    Eduardo Santini-Araujo

  2. Laboratory of Orthopaedic Pathology, Buenos Aires, Argentina

    Ricardo K. Kalil

  3. Pathology, BBTEAM Pathology, Bologna, Italy

    Franco Bertoni

  4. Department of Pathology, Kyung Hee University Hospital, Seoul, Korea (Republic of)

    Yong-Koo Park

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Na, K., Park, YK. (2020). Basic and Ancillary Techniques in Bone Pathology. In: Santini-Araujo, E., Kalil, R.K., Bertoni, F., Park, YK. (eds) Tumors and Tumor-Like Lesions of Bone. Springer, Cham. https://doi.org/10.1007/978-3-030-28315-5_5

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  • DOI: https://doi.org/10.1007/978-3-030-28315-5_5

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-28314-8

  • Online ISBN: 978-3-030-28315-5

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