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Human Induced Pluripotent Stem Cells as Platform for Functional Examination of Cardiovascular Genetics in a Dish

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Genetic Causes of Cardiac Disease

Part of the book series: Cardiac and Vascular Biology ((Abbreviated title: Card. vasc. biol.,volume 7))

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Abstract

The three most ground-breaking developments in biomedical research in the last decades may have been the generation of human induced pluripotent stem cells (hiPSCs) from diseased and healthy individuals, the discovery of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) genome editing system, and the possibility to identify disease-related genetic variants in human populations in genome-wide association studies (GWAS) or by modern sequencing methods. Although these highly complementary fields have evolved rapidly in the last decade and already profoundly changed our understanding of cardiovascular genetics, there is tremendous potential to further merge these technologies. This chapter aims to summarize the state of the art with respect to the use of hiPSCs to model cardiovascular genetic diseases, to pinpoint challenges and limitations of this technology, and to outline potential strategies to overcome these by the implementation of evolving genome editing technology and human genetic research tools.

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Authors and Affiliations

  1. Cardiology Division, First Department of Medicine, Klinikum rechts der Isar – Technical University of Munich, Munich, Germany

    Ralf Dirschinger, Tatjana Dorn & Alessandra Moretti

  2. Munich Heart Alliance, Munich, Germany

    Alessandra Moretti

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  1. Ralf Dirschinger
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  1. IIEG, University of Lubeck, Lübeck, Germany

    Jeanette Erdmann

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    Alessandra Moretti

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Dirschinger, R., Dorn, T., Moretti, A. (2019). Human Induced Pluripotent Stem Cells as Platform for Functional Examination of Cardiovascular Genetics in a Dish. In: Erdmann, J., Moretti, A. (eds) Genetic Causes of Cardiac Disease. Cardiac and Vascular Biology, vol 7. Springer, Cham. https://doi.org/10.1007/978-3-030-27371-2_10

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