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New Materials for the Construction of Electrochemical Cell-Based Biosensors

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Handbook of Cell Biosensors

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Abstract

The development of new biosensors with applications in various domains represents a contemporary challenge that it is extensively studied. The elaboration of cell-based biosensors has been a major outbreak in the sensing field due to their high sensitivity and specificity doubled by high stability and catalytic activity of the enzymatic systems included in the immobilized living cells. The major drawback of the conventional enzymatic biosensors represented by the loss of the enzymatic activity was eliminated by the immobilization of whole-cells on sensing platforms while maintaining their stability. The selection of the whole-cell, either mammalian or microorganism, orientates the detection towards heterogeneous compounds such as heavy metals, pollutants, foodborne pathogens, and biomedical biomarkers. By keeping the enzymes in a cellular environment, the enzymatic turnover was facilitated and their catalytic activity loss was greatly reduced. A key point is represented by the selection of suitable platforms that can ensure the stability of the cell and allow the monitoring of the metabolic transfer with the extracellular environment. Depending on the end-application, the cells could be coated with a protective polymeric layer or they can be immobilized in different biocompatible polymers. Moreover, adding carbon-based and/or metallic nanoparticles, their stability and catalytic properties are highly improved. The future trends in sensing strategies involve the association between different nanomaterials, miniaturization and the development of out-of the box sensing devices with improved analytical performances. The present chapter discusses multiple approaches for the elaboration of nanohybrid platforms, along with their advantages and limitations and it also underlines the materials used for designing wearable sensing devices.

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Acknowledgments

This work was supported by the Romanian National Authority for Scientific Research and Innovation, CNCS/CCCDI-UEFISCDI, project number PN-III-P1-1.2-PCCDI2017-0407 (INTELMAT).

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy Iuliu Hatieganu Cluj-Napoca, Cluj-Napoca, Romania

    Andreea Cernat, Bianca Ciui, Mihaela Tertis & Cecilia Cristea

  2. Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, Oradea, Romania

    Luminita Fritea

Authors
  1. Andreea Cernat
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  2. Bianca Ciui
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  3. Luminita Fritea
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  4. Mihaela Tertis
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  5. Cecilia Cristea
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Corresponding author

Correspondence to Cecilia Cristea .

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Editors and Affiliations

  1. UMR CNRS 6144 GEPEA, Institut Universitaire de Technologie, La Roche sur Yon, France

    Gérald Thouand

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    Shimshon Belkin

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    Sylvia Daunert

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    Paul Freemont

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    Julie Hermans

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    Isao Karube

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    Sylvain Martel

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    Elisa Michelini

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    Aldo Roda

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    Aldo Roda

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    Sylvia Daunert

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    Elisa Michelini

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    Sylvain Martel

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Cernat, A., Ciui, B., Fritea, L., Tertis, M., Cristea, C. (2022). New Materials for the Construction of Electrochemical Cell-Based Biosensors. In: Thouand, G. (eds) Handbook of Cell Biosensors. Springer, Cham. https://doi.org/10.1007/978-3-030-23217-7_138

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