Abstract
The FLIO device is based on a Heidelberg Spectralis device. Retinal autofluorescence is excited with a 470 nm laser, and fluorescence emission is detected in two distinct wavelength channels by a time-correlated single photon counting system. An infrared camera is used for eye tracking. Imaging of a 30° field of view on the posterior pole is performed. Image data is further processed and analyzed using Becker & Hickl software.
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References
Dysli C, et al. Fluorescence lifetime imaging ophthalmoscopy. Prog Retin Eye Res. 2017;60:120–43.
Schweitzer D, et al. [Time-correlated measurement of autofluorescence. A method to detect metabolic changes in the fundus]. Ophthalmologe. 2002;99(10):774–779.
Schweitzer D, et al. Basic investigations for 2-dimensional time-resolved fluorescence measurements at the fundus. Int Ophthalmol. 2001;23(4–6):399–404.
Schweitzer D, Kolb A, Hammer M. Autofluorescence lifetime measurements in images of the human ocular fundus. In: Proc SPIE, vol. 4432; 2001. p. 29–39.
Dysli C, et al. Quantitative analysis of fluorescence lifetime measurements of the macula using the fluorescence lifetime imaging ophthalmoscope in healthy subjects. Invest Ophthalmol Vis Sci. 2014;55(4):2106–13.
Becker W. The bh TCSPC handbook. 6th ed. Berlin: Becker & Hickl GmbH; 2014.
Becker W, et al. Fluorescence lifetime imaging by time-correlated single-photon counting. Microsc Res Tech. 2004;63(1):58–66.
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Dysli, C., Zinkernagel, M., Wolf, S. (2019). The Fluorescence Lifetime Imaging Ophthalmoscope. In: Zinkernagel, M., Dysli, C. (eds) Fluorescence Lifetime Imaging Ophthalmoscopy. Springer, Cham. https://doi.org/10.1007/978-3-030-22878-1_5
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DOI: https://doi.org/10.1007/978-3-030-22878-1_5
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