Abstract
Microsphere-assisted microscopy is a new two-dimensional super- resolution imaging technique, which allows the diffraction limit to be overcome by introducing a transparent microsphere in a classical optical microscope. This super-resolution technique makes it possible to reach a lateral resolution of up to one hundred nanometres. Furthermore, microsphere-assisted microscopy distinguishes itself from others by being able to perform label-free and full-field acquisitions and requires only slight modifications of a classical white light microscope. Extended to three-dimensional surface measurement through interference microscopy which has the advantage of providing a high-axial sensitivity, super-resolution topography or the volume distribution of objects can thus be reconstructed depending on the interference method employed. This chapter first presents a brief history of optical microscopy and recent advances in optical nanoscopy. Then, the super-resolution phenomenon through microspheres is introduced and its performance is described. Finally, the combination of optical interferometry with nanoscopy based on microspheres, giving microsphere-assisted interference microscopy, is exposed.
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Acknowledgements
The author would like to thank the members of IPP research team of the ICube Laboratory (Strasbourg, France) for their contributions, specially A. Leong-Hoi for her PhD thesis work on super-resolution optical imaging and P. Twardowski for his useful discussions and advice. Moreover, we thank all partners who have directly or indirectly contributed to the linked associated research projects.
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Perrin, S., Lecler, S., Montgomery, P. (2019). Microsphere-Assisted Interference Microscopy. In: Astratov, V. (eds) Label-Free Super-Resolution Microscopy. Biological and Medical Physics, Biomedical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-030-21722-8_17
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