Abstract
The remarkable physical and chemical properties of pullulan, especially the biodegradability, biocompatibility, and nontoxicity, have been exploited in the past few decades and adapted in order to design more efficient drug delivery systems. This polysaccharide itself and its derivatives, which possess more reactive functional groups generated by functionalization of pullulan, were able to form conjugates or complexes with a variety of drugs, especially with hydrophobic drugs. By modulating the hydrophilic-hydrophobic balance in the support macromolecule structure and favoring various types of physical interactions between drug and carrier, the researchers attempted to optimize the charging and subsequent transport of drugs to target cells such as liver cell receptors or cancer cells.
In this chapter, beside the pullulan-based systems with antibacterial, antifungal, antitumor, antioxidant, anti-inflammatory, immunomodulatory, antilipidemic, or antiglycemic properties, other pharmaceutical formulations potentially useful to treat heart or bone diseases were reviewed. All studies highlighted the versatility of pullulan derivatives to form micelles, films, hydrogels, microparticles, and nanoparticles. Also, the results from in vivo and in vitro tests of cytotoxicity and the profiles of drug release from these carriers were encouraging such that the usage of pullulan polysaccharides for the future medical applications remains an open field.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Balasso A, Salmaso S, Pontisso P, Rosato A, Quarta S, Malfranti A, Mastrotto F, Caliceti P (2017) Re-programming pullulan for targeting and controlled release of doxorubicin to the hepatocellular carcinoma cells. Eur J Pharm Sci 103:104–115. https://doi.org/10.1016/j.ejps.2017.02.016
Bishwambhar M, Vuppu S (2012) Release study of naproxen, a modern drug from pH sensitive pullulan acetate microsphere. Int J Drug Dev Res 4:184–191
Bowman SM, Free SJ (2006) The structure and synthesis of the fungal cell wall. Bioassay 28:799–808. https://doi.org/10.1002/bies.20441
Chen L, Wang X, Ji F, Bao Y, Wang J, Wang X, Guo L, Li Y (2015) New bifunctional-pullulan-based micelles with good biocompatibility for efficient co-delivery of cancer-suppressing p53 gene and doxorubicin to cancer cells. RSC Adv 5:94719–94731. https://doi.org/10.1039/c5ra17139c
Chen L, Ji F, Bao Y, Xia J, Guo L, Wang J, Li Y (2017) Biocompatible cationic pullulan-g-desoxycholic acid-g-PEI micelles used to co-deliver drug and gene for cancer therapy. Mater Sci Eng C 70:418–429. https://doi.org/10.1016/j.msec.2016.09.019
Cheng K-C, Demirci A, Catchmark JM (2011) Pullulan: biosynthesis, production, and applications. Appl Microbiol Biotechnol 92:29–44. https://doi.org/10.1016/j.msec.2016.09.019
Choi JM, Lee B, Jeong D, Park KH, Choi E-J, Jeon Y-J, Dindulkar SD, Cho E, Do SH, Lee K, Lee I-S, Park S, Jun B-H, Yu J-H, Jung S (2017) Characterization and regulated naproxen release of hydroxypropyl cyclosophoraose-pullulan microspheres. J Ind Eng Chem 48:108–118. https://doi.org/10.1016/j.jiec.2016.12.026
Constantin M, Bucatariu S, Stoica I, Fundueanu G (2017) Smart nanoparticles based on pullulan-g-poly(N-isopropylacrylamide) for controlled delivery of indomethacin. Int J Biol Macromol 94:698–708. https://doi.org/10.1016/j.ijbiomac.2016.10.064
Cristescu R, Popescu C, Popescu AC, Socol G, Mihailescu I, Caraene G, Albulescu R, Buruiana T, Chrisey D (2012) Pulsed laser processing of functionalized polysaccharides for controlled release drug delivery systems: Functionalized polysaccharides processed for drug delivery. In: Vaseashta A et al (eds) Technological innovations in sensing and detection of chemical, biological, radiological, nuclear threats and ecological terrorism, NATO science for peace and security series A: chemistry and biology. Springer Science Business Media B.V., Heidelberg, pp 231–236
de Arce VA, Ferreira LM, Stangarlin MFL, da Silva CB, Rolim CMB, Cruz L (2014) Novel Pullulan-Eudragit S100 blend microparticles for oral delivery of risedronate: formulation, in vitro evaluation and tableting of blend microparticles. Mater Sci Eng C 38:212–217. https://doi.org/10.1016/j.msec.2014.02.003
de Lima JA, Paines TC, Motta MH, Weber WB, Dos Santos SS, Cruz L, da Silva CDB (2017) Novel Pemulen/pullulan blended hydrogel containing clotrimazole-loaded cationic nanocapsules: Evaluation of mucoadhesion and vaginal permeation. Mater Sci Eng C 79:886–893. https://doi.org/10.1016/j.msec.2017.05.030
Di Meo C, Montanari E, Manzi L, Villani C, Coviello T, Matricardi P (2015) Highly versatile nanohydrogel platform based on riboflavin-polysaccharide derivatives useful in the development of intrinsically fluorescent and cytocompatible drug carriers. Carbohydr Polym 115:502–509. https://doi.org/10.1016/j.carbpol.2014.08.107
Dickinson BC, Chang CJ (2011) Chemistry and biology of reactive oxygen species in signaling or stress responses. Nat Chem Biol 7:504–511. https://doi.org/10.1038/nchembio.607
D’Souza AA, Jain P, Galdhar CN, Samad A, Degani MS, Devarajan PV (2013) Comparative in silico-in vivo evaluation of ASGP-R ligands for hepatic targeting of curcumin Gantrez nanoparticles. AAPS J 15:696–706. https://doi.org/10.1208/s12248-013-9474-6
El-Malah Y, Nazzal S (2013) Real-time disintegration analysis and D-optimal experimental design for the optimization of diclofenac sodium fast-dissolving films. Pharm Dev Technol 18:1355–1360. https://doi.org/10.3109/10837450.2012.700936
Flores FC, Rosso RS, Cruz L, Beck RCR, Silva CB (2017) An innovative polysaccharide nanobased nail formulation for improvement of onychomycosis treatment. Eur J Pharm Sci 100:56–63. https://doi.org/10.1016/j.ejps.2016.12.043
Ganeshkumar M, Ponrasu T, Subamekala MK, Janani M, Suguna L (2016) Curcumin loaded on pullulan acetate nanoparticles protects the liver from damage induced by DEN. RSC Adv 6:5599–5610. https://doi.org/10.1039/C5RA18989F
Garhwal R, Shady SF, Ellis EJ, Leahy CD, McCarthy SP, Crawford KS, Gaines P (2012) Sustained ocular delivery of ciprofloxacin using nanospheres and conventional contact lens materials. Investig Ophthalmol Vis Sci 53:1341–1352. https://doi.org/10.1167/iovs.11-8215
Grigoras AG (2016) A review on medical applications of poly(N-vinylcarbazole) and its derivatives. Int J Polym Mater Polym Biomater 65:888–900. https://doi.org/10.1080/00914037.2016.1180613
Guhagarkar SA, Gaikwad RV, Samad A, Malshe VC, Devarajan PV (2010) Polyethylene sebacate-doxorubicin nanoparticles for hepatic targeting. Int J Pharm 401:113–122. https://doi.org/10.1016/j.ijpharm.2010.09.012
Guhagarkar SA, Shah D, Patel MD, Sathaye SS, Devarajan PV (2015) Polyethylene sebacate-Silymarin nanoparticles with enhanced hepatoprotective activity. J Nanosci Nanotechnol 15:4090–4093. https://doi.org/10.1166/jnn.2015.9518
Hassanzadeh F, Varshosaz J, Khodarahmi GA, Rostami M, Hassanzadeh F (2016) Biotin-encoded pullulan-retinoic acid engineered nanomicelles: preparation, optimization and in vitro cytotoxicity assessment in MCF-7 cells. Indian J Pharm Sci 78:557–565. https://doi.org/10.4172/pharmaceutical-sciences.1000153
Hassanzadeh F, Mehdifar M, Varshosaz J, Khodarahmi GA, Rostami M (2018) Folic acid targeted polymeric micelles based on tocopherol succinate- pullulan as an effective carrier for epirubicin: preparation, characterization and in-vitro cytotoxicity assessment. Curr Drug Deliv 15:235–246. https://doi.org/10.2174/1567201814666170602074149
Hong G-Y, Jeong Y-I, Lee SJ, Lee E, Oh JS, Lee HC (2011) Combination of paclitaxel- and retinoic acid-incorporated nanoparticles for the treatment of CT-26 colon carcinoma. Arch Pharm Sci Res 34:407–411. https://doi.org/10.1007/s12272-011-0308-8 https://www.drugbank.ca/drugs
Huang L, Wang Y, Ling X, Chaurasiya B, Yang C, Du Y, Tu J, Xiong Y, Sun C (2017) Efficient delivery of paclitaxel into ASGPR over-expressed cancer cells using reversibly stabilized multifunctional pullulan nanoparticles. Carbohydr Polym 159:178–187. https://doi.org/10.1016/j.carbpol.2016.11.094
Huo M, Zhang Y, Zhou J, Zou A, Yu D, Wu Y, Li J, Li H (2010) Synthesis and characterization of low-toxic amphiphilic chitosan derivatives and their application as micelle carrier for antitumor drug. Int J Pharm 394:162–173. https://doi.org/10.1016/j.ijpharm.2010.05.001
Jeong YI, Na HS, Oh JS, Choi KC, Song CE, Lee HC (2006) Adriamycin release from self-assembling nanospheres of poly(DL-lactide-co-glycolide)-grafted pullulan. Int J Pharm 322:154–160. https://doi.org/10.1016/j.ijpharm.2006.05.020
Jung Y-S, Park W, Na K (2013) Temperature-modulated noncovalent interaction controllable complex for the long-term delivery of etanercept to treat rheumatoid arthritis. J Control Release 171:143–151. https://doi.org/10.1016/j.jconrel.2013.07.012
Kang J-H, Tachibana Y, Obika S, Harada-Shiba M, Yamaoka T (2012) Efficient reduction of serum cholesterol by combining a liver-targeted gene delivery system with chemically modified apolipoprotein B siRNA. J Control Release 163:119–124. https://doi.org/10.1016/j.jconrel.2012.08.030
Kratz F, Warneckje K, Riebessel K, Rodrigues PCA (2002) Anticancer drug conjugates with macromolecular carriers. In: Dumitriu S (ed) Polymeric biomaterials. Marcel Dekker, Inc., New York/Basel, pp 851–895
Krull SM, Ma Z, Li M, Dave RN, Bilgili E (2016) Preparation and characterization of fast dissolving pullulan films containing BCS class II drug nanoparticles for bioavailability enhancement. Drug Dev Ind Pharm 42:1073–1085. https://doi.org/10.3109/03639045.2015.1107094
Lee SJ, Hong G-Y, Jeong Y-I, Kang MS, Oh JS, Song C-E, Lee HC (2012) Paclitaxel-incorporated nanoparticles of hydrophobized polysaccharide and their antitumor activity. Int J Pharm 433:121–128. https://doi.org/10.1016/j.ijpharm.2012.04.048
Lee J, Jeong D, Seo S, Na K (2013) Biodegradable nanogel based on all-trans retinoic acid/pullulan conjugate for anti-cancer drug delivery. J Pharm Investig 43:63–69. https://doi.org/10.1007/s40005-013-0055-6
Lee SJ, Shim Y-H, Oh J-S, Jeong Y-I, Park I-K, Lee HC (2015) Folic-acid-conjugated pullulan/poly(DL-lactide-co-glycolide) graft copolymer nanoparticles for folate-receptor-mediated drug delivery. Nanoscale Res Lett 10:1–11. https://doi.org/10.1186/s11671-014-0706-1
Lee IW, Li J, Chen X, Park HJ (2017) Fabrication of electrospun antioxidant nanofibers by rutin-pluronic solid dispersions for enhanced solubility. J Appl Polym Sci 134:44859. https://doi.org/10.1002/app.44859
Li J, Huo M, Wang J, Zhou J, Mohammad JM, Zhang Y, Zhu Q, Waddad AY, Zhang Q (2012) Redox-sensitive micelles self-assembled from amphiphilic hyaluronic acid-deoxycholic acid conjugates for targeted intracellular delivery of paclitaxel. Biomaterials 33:2310–2320. https://doi.org/10.1016/j.biomaterials.2011.11.022
Li F, Zhang H, Gu C, Fan L, Qiao Y, Tao Y, Cheng C, Wu H, Yi J (2013) Self-assembled nanoparticles from folate-decorated maleilated pullulan-doxorubicin conjugate for improved drug delivery to cancer cells. Polym Int 62:165–171. https://doi.org/10.1002/pi.4272
Li H, Cui Y, Liu J, Bian S, Liang J, Fan Y, Zhang X (2014) Reduction breakable cholesteryl pullulan nanoparticles for targeted hepatocellular carcinoma chemotherapy. J Mater Chem B 2:3500–3510. https://doi.org/10.1039/C4TB00321G
Li H, Cui Y, Sui J, Liang J, Fan Y, Zhang X (2015a) Efficient delivery of DOX to nuclei of hepatic carcinoma cells in the subcutaneous tumor model using pH-sensitive pullulan-DOX conjugates. ACS Appl Mater Interfaces 7:15855–15865. https://doi.org/10.1021/acsami.5b03150
Li H, Sun Y, Liang J, Fan Y, Zhang X (2015b) pH-sensitive pullulan–DOX conjugate nanoparticles for co-loading PDTC to suppress growth and chemoresistance of hepatocellular carcinoma. J Mater Chem B 41:8070–8078. https://doi.org/10.1039/C5TB01210D
Liu J, Jo J-I, Kawai Y, Aoki I, Tanaka C, Yamamoto M, Tabata Y (2012) Preparation of polymer-based multimodal imaging agent to visualize the process of bone regeneration. J Control Release 157(3):398–405. https://doi.org/10.1016/j.jconrel.2011.09.090
Liu Y, Wang Y, Zhang C, Zhou P, Liu Y, An T, Sun D, Zhang N, Wang Y (2014) Core-shell nanoparticles based on pullulan and poly(β-amino)ester for hepatoma-targeted codelivery of gene and chemotherapy agent. ACS Appl Mater Interfaces 6:18712–18720. https://doi.org/10.1021/am504203x
Lv Q-Y, Li X-Y, Shen B-D, Dai L, Xu H, Chen C-Y, Yuan H-L, Han J (2015) A solid phospholipid-bile salts-mixed micelles based on the fast dissolving oral films to improve the oral bioavailability of poorly water-soluble drugs. J Nanopart Res 16:2455. https://doi.org/10.1007/s11051-014-2455-6
Mitha AT, Rekha MR (2014) Multifunctional polymeric nanoplexes for anticancer co-delivery of p53 and mitoxantrone. J Mater Chem B 2:8005–8016. https://doi.org/10.1039/C4TB01298D
Mocanu G, Nichifor M, Picton L, About-Jaudet E, Le Cerf D (2014) Preparation and characterization of anionic pullulan thermoassociative nanoparticles for drug delivery. Carbohydr Polym 111:892–900. https://doi.org/10.1016/j.carbpol.2014.05.037
Mohamed Wali AR, Zhou J, Ma S, He Y, Yue D, Tang JZ, Gu Z (2017) Tailoring the supramolecular structure of amphiphilic glycopolypeptide analogue toward liver targeted drug delivery systems. Int J Pharm 525(1):191–202. https://doi.org/10.1016/j.ijpharm.2017.04.009
Moon S, Yang S-G, Na K (2011) An acetylated polysaccharide-PTFE membrane-covered stent for the delivery of gemcitabine for treatment of gastrointestinal cancer and related stenosis. Biomaterials 32:3603–3610. https://doi.org/10.1016/j.biomaterials.2011.01.070
Parejiya PB, Patel RC, Mehta DM, Shelat PK, Barot BS (2013) Quick dissolving films of nebivolol hydrochloride: Formulation and optimization by a simplex lattice design. J Pharm Investig 43:343–351. https://doi.org/10.1007/s40005-013-0080-5
Patel V, Patel J (2012) Pullulan acetate controlled-release biodegradable microsphere containing a biologically active agent: Preparation, characterization and in vitro experiments. Asian J Pharm Clin Res 5:143–147
Prajapati VD, Jani GK, Khanda SM (2013) Pullulan: an exopolysaccharide and its various applications. Carbohydr Polym 95:540–549. https://doi.org/10.1016/j.carbpol.2013.02.082
Pranatharthiharan S, Patel MD, Malshe VC, Pujari V, Gorakshakar A, Madkaikar M, Ghosh K, Devarajan PV (2017) Asialoglycoprotein receptor targeted delivery of doxorubicin nanoparticles for hepatocellular carcinoma. Drug Deliv 24:20–29. https://doi.org/10.1080/10717544.2016.1225856
Priya SS, Rekha MR (2017) Redox sensitive cationic pullulan for efficient gene transfection and drug retention in C6 glioma cells. Int J Pharm 530:401–414. https://doi.org/10.1016/j.ijpharm.2017.08.004
Reddy NS, Kumar CBM, Ramesh A, Chandrashekhar MS (2011) Development and in vitro evaluation of gastro retentive matrix tablets: an approach using natural gums and polymers. Res J Pharm, Biol Chem Sci 2:90–107
Santhosh Kumar B, Ganesh Kumar M, Suguna L, Sastry TP, Mandal AB (2012) Pullulan acetate nanoparticles based delivery system for hydrophobic drug. Int J Pharm Bio Sci 3:24–32
Sarika PR, James NR, Nishna N, Anil Kumar PR, Raj DK (2015) Galactosylated pullulan-curcumin conjugate micelles for site specific anticancer activity to hepatocarcinoma cells. Colloids Surf B: Biointerfaces 133:347–355. https://doi.org/10.1016/j.colsurfb.2015.06.020
Scomparin A, Salmaso S, Bersani S, Satchi-Fainaro R, Caliceti P (2011) Novel folated and non-folated pullulan bioconjugates for anticancer drug delivery. Eur J Pharm Sci 42:547–558. https://doi.org/10.1016/j.ejps.2011.02.012
Scomparin A, Salmaso S, Eldar-Boock A, Ben-Shushan D, Ferber S, Tiram G, Shmeeda H, Landa-Rouben N, Leor J, Caliceti P, Gabizon A, Satchi-Fainaro R (2015) A comparative study of folate receptor-targeted doxorubicin delivery systems: dosing regimens and therapeutic index. J Control Release 208:106–120. https://doi.org/10.1016/j.jconrel.2015.04.009
Seo S, Lee C-S, Jung Y-S, Na K (2012) Thermo-sensitivity and triggered drug release of polysaccharide nanogels derived from pullulan-g-poly(L-lactide) copolymers. Carbohydr Polym 87:1105–1111. https://doi.org/10.1016/j.carbpol.2011.08.061
Shen S, Li H, Yang W (2014) The preliminary evaluation on cholesterol-modified pullulan as a drug nanocarrier. Drug Deliv 21:501–508. https://doi.org/10.3109/10717544.2014.895068
Singh RS, Saini GK, Kennedy JF (2008) Pullulan: microbial sources, production and applications. Carbohydr Polym 73:515–531. https://doi.org/10.1016/j.carbpol.2008.01.003
Singh RS, Kaura N, Kennedy JF (2015) Pullulan and pullulan derivatives as promising biomolecules for drug and gene targeting. Carbohydr Polym 123:190–207. https://doi.org/10.1016/j.carbpol.2015.01.032
Singh RS, Kaura N, Ranab V, Kennedy JF (2017) Pullulan: a novel molecule for biomedical applications. Carbohydr Polym 171:102–121. https://doi.org/10.1016/j.carbpol.2017.04.089
Sui J, Cui Y, Cai H, Bian S, Xu Z, Zhou L, Sun Y, Liang J, Fan Y, Zhang X (2017) Synergistic chemotherapeutic effect of sorafenib-loaded pullulan-Dox conjugate nanoparticles against murine breast carcinoma. Nanoscale 9:2755–2767. https://doi.org/10.1039/c6nr09639e
Sushmitha S, Priyanka SR, Mohan Krishna L, Srinavasa Murthy M (2014) Formulation and evaluation of mucoadhesive fast melt-away wafers using selected polymers. Res. J. Pharm. Technol. 7:176–180
Tang HB, Li L, Chen H, Zhou ZM, Chen HL, Li XM, Liu LR, Wang YS, Zhang QQ (2010) Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier. Drug Deliv 17:552–558. https://doi.org/10.3109/10717544.2010.490250
Tao X, Zhang Q, Ling K, Chen Y, Yang W, Gao F, Shi G (2012) Effect of pullulan nanoparticle surface charges on HSA complexation and drug release behavior of HSA-bound nanoparticles. PLoS One 7:e49304. https://doi.org/10.1371/journal.pone.0049304
Tuncay Tanriverdi S, Hilmiolu Polat S, Yeşim Metin D, Kandilolu G, Ozer O (2016) Terbinafine hydrochloride loaded liposome film formulation for treatment of onychomycosis: in vitro and in vivo evaluation. J Liposome Res 26:163–173. https://doi.org/10.3109/08982104.2015.1067892
Vishwanath B, Shivakumar HR, Sheshappa RK, Ganesh S, Prasad P, Guru GS, Bhavya BB (2012a) In-vitro release study of metoprolol succinate from the bioadhesive films of pullulan-polyacrylamide blends. Int J Polym Mater Polym Biomater 61:300–307. https://doi.org/10.1080/00914037.2011.584227
Vishwanath B, Shivakumar HR, Sheshappa RK, Ganesh S, Bhavya BB (2012b) Influence of blending of chitosan and pullulan on their drug release behavior: an in-vitro study. Int J Pharm Pharm Sci 4:313–317
Vora L, Tyagi M, Patel K, Gupta S, Vavia P (2014) Self-assembled nanocomplexes of anionic pullulan and polyallylamine for DNA and pH-sensitive intracellular drug delivery. J Nanopart Res 16:2781. https://doi.org/10.1007/s11051-014-2781-8
Wang J, Cui S, Bao Y, Xing J, Hao W (2014a) Tocopheryl pullulan-based self-assembling nanomicelles for anti-cancer drug delivery. Mater Sci Eng C 43:614–621. https://doi.org/10.1016/j.msec.2014.07.066
Wang X, Wang J, Bao Y, Wang B, Wang X, Chen L (2014b) Novel reduction-sensitive pullulan-based micelles with good hemocompatibility for efficient intracellular doxorubicin delivery. RSC Adv 4:60064–60074. https://doi.org/10.1039/C4RA12276C
Wang Y, Liu Y, Liu Y, Wang Y, Wu J, Li R, Yang J, Zhang N (2014c) pH-sensitive pullulan-based nanoparticles for intracellular drug delivery. Polym Chem 5:423–432. https://doi.org/10.1039/C3PY00817G
Wang M, Huang M, Wang J, Ye M, Deng Y, Li H, Qian W, Zhu B, Zhang Y, Gong R (2016) Facile one-pot synthesis of self-assembled folate-biotin-pullulan nanoparticles for targeted intracellular anticancer drug delivery J Nanomater Article ID 5752921, 10 pages. https://doi.org/10.1155/2016/5752921
Yang W-Z, Chen H-L, Gao F-P, Chen M-M, Li X-M, Zhang M-M, Zhang Q-Q, Liu L-R, Jiang Q, Wang Y-S (2010) Self-aggregated nanoparticles of cholesterol-modified pullulan conjugate as a novel carrier of mitoxantrone. Current Nanoscience 6:298–306. https://doi.org/10.2174/157341310791171153
Yang W, Wang M, Ma L, Li H, Huang L (2014) Synthesis and characterization of biotin modified cholesteryl pullulan as a novel anticancer drug carrier. Carbohydr Polym 99:720–727. https://doi.org/10.1016/j.carbpol.2013.09.013
Yim H, Park S-J, Bae YH, Na K (2013) Biodegradable cationic nanoparticles loaded with an anticancer drug for deep penetration of heterogeneous tumors. Biomaterials 34:7674–7682. https://doi.org/10.1016/j.biomaterials.2013.06.058
Yuan R, Zheng F, Zhong S, Tao X, Zhang Y, Gao F, Yao F, Chen J, Chen Y, Shi G (2014) Self-assembled nanoparticles of glycyrrhetic acid-modified pullulan as a novel carrier of curcumin. Molecules 19:13305–13318. https://doi.org/10.3390/molecules190913305
Zhang H-Z, Li X-M, Gao F-P, Liu LR, Zhou Z-M, Zhang Q-Q (2010) Preparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug delivery. Drug Deliv 17:48–57. https://doi.org/10.3109/10717540903508979
Zhang H, Li F, Yi J, Gu C, Fan L, Qiao Y, Tao Y, Cheng C, Wu H (2011) Folate-decorated maleilated pullulan-doxorubicin conjugate for active tumor-targeted drug delivery. Eur J Pharm Sci 42:517–526. https://doi.org/10.1016/j.ejps.2011.02.006
Zhang C, An T, Wang D, Wan G, Zhang M, Wang H, Zhang S, Li R, Yang X, Wang Y (2016) Stepwise pH-responsive nanoparticles containing charge-reversible pullulan-based shells and poly(β-amino ester)/poly(lactic-co-glycolic acid) cores as carriers of anticancer drugs for combination therapy on hepatocellular carcinoma. J Control Release 226:193–204. https://doi.org/10.1016/j.jconrel.2016.02.030
Zhu W, Romanski FS, Meng X, Mitra S, Tomassone MS (2011) Atomistic simulation study of surfactant and polymer interactions on the surface of a fenofibrate crystal. Eur J Pharm Sci 42:452–461. https://doi.org/10.1016/j.ejps.2011.01.009
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Grigoras, A.G. (2019). Drug Delivery Systems Based on Pullulan Polysaccharides and Their Derivatives. In: Arora, D., Sharma, C., Jaglan, S., Lichtfouse, E. (eds) Pharmaceuticals from Microbes. Environmental Chemistry for a Sustainable World, vol 26. Springer, Cham. https://doi.org/10.1007/978-3-030-01881-8_4
Download citation
DOI: https://doi.org/10.1007/978-3-030-01881-8_4
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-01880-1
Online ISBN: 978-3-030-01881-8
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)