Abstract
The adult hematopoietic system is repopulated in its entirety from a rare cell type known as hematopoietic stem cells (HSCs) that reside in the marrow space throughout the skeletal system. Here we describe the isolation and identification of HSCs both phenotypically and functionally.
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References
Osawa M et al (1996) Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell. Science 273(5272):242–245
Purton LE, Scadden DT (2007) Limiting factors in murine hematopoietic stem cell assays. Cell Stem Cell 1(3):263–270
Matsuzaki Y et al (2004) Unexpectedly efficient homing capacity of purified murine hematopoietic stem cells. Immunity 20(1):87–93
Yilmaz OH, Kiel MJ, Morrison SJ (2006) SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity. Blood 107(3):924–930
van Os R, Kamminga LM, de Haan G (2004) Stem cell assays: something old, something new, something borrowed. Stem Cells 22(7):1181–1190
Okada S et al (1992) In vivo and in vitro stem cell function of c-kit- and Sca-1-positive murine hematopoietic cells. Blood 80(12):3044–3050
Spangrude GJ, Heimfeld S, Weissman IL (1988) Purification and characterization of mouse hematopoietic stem cells. Science 241(4861):58–62
Morrison SJ, Weissman IL (1994) The long-term repopulating subset of hematopoietic stem cells is deterministic and isolatable by phenotype. Immunity 1(8):661–673
Morrison SJ et al (1997) Identification of a lineage of multipotent hematopoietic progenitors. Development 124(10):1929–1939
Bradley TR, Metcalf D (1966) The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci 44(3):287–299
Ploemacher RE et al (1989) An in vitro limiting-dilution assay of long-term repopulating hematopoietic stem cells in the mouse. Blood 74(8):2755–2763
Ploemacher RE et al (1991) Use of limiting-dilution type long-term marrow cultures in frequency analysis of marrow-repopulating and spleen colony-forming hematopoietic stem cells in the mouse. Blood 78(10):2527–2533
Sutherland HJ et al (1989) Characterization and partial purification of human marrow cells capable of initiating long-term hematopoiesis in vitro. Blood 74(5):1563–1570
Ford CE et al (1956) Cytological identification of radiation-chimaeras. Nature 177(4506):452–454
McCulloch EA, Till JE (1960) The radiation sensitivity of normal mouse bone marrow cells, determined by quantitative marrow transplantation into irradiated mice. Radiat Res 13:115–125
Szilvassy SJ et al (1990) Quantitative assay for totipotent reconstituting hematopoietic stem cells by a competitive repopulation strategy. Proc Natl Acad Sci U S A 87(22):8736–8740
Acknowledgment
This work was supported by NIH grants 1R01DK081843, 1R01CA166280, 5R01DK076876, 1U01AI107276, U19AI091036-01, and DoD grant BM110106 to L.M.C.
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Frisch, B.J., Calvi, L.M. (2014). Hematopoietic Stem Cell Cultures and Assays. In: Hilton, M. (eds) Skeletal Development and Repair. Methods in Molecular Biology, vol 1130. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-989-5_24
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DOI: https://doi.org/10.1007/978-1-62703-989-5_24
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-988-8
Online ISBN: 978-1-62703-989-5
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