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A Growth Quantification Assay for Hyaloperonospora arabidopsidis Isolates in Arabidopsis thaliana

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Plant-Pathogen Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1127))

Abstract

There is a considerable interest in determining the role of individual oomycete effectors in promoting disease. Widely used strategies are based on manipulating effector-expression levels in the pathogen and by over-expressing particular effectors in the host by genetic transformation. In the case of the oomycete, Hyaloperonospora arabidopsidis (Hpa) genetic manipulation is not yet possible, so over-expression of predicted effectors in stably transformed Arabidopsis lines is used to investigate their capability for promoting virulence. Here, we describe a technique for quantifying pathogen growth based on the counting of asexual reproductive structures called sporangiophores in the compatible interaction between the Hpa isolate Noks1 and the Col-0 Arabidopsis accession.

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Acknowledgments

D.F.A.T. and J.S. are funded by the UK Biotechnology and Biological Sciences Research Council grant BB/G015066/1 to J.L.B.

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Authors and Affiliations

  1. School of Life Sciences, Warwick University, Gibbet Hill Campus, Coventry, CV4 7 AL, United Kingdom

    Daniel F. A. Tomé, Jens Steinbrenner & Jim L. Beynon

  2. Warwick Systems Biology, Warwick University, Coventry, United Kingdom

    Jim L. Beynon

Authors
  1. Daniel F. A. Tomé
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  2. Jens Steinbrenner
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  3. Jim L. Beynon
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Corresponding author

Correspondence to Jim L. Beynon .

Editor information

Editors and Affiliations

  1. Dundee Effector Consortium, Cell and Molecular Sciences, The James Hutton Institute, Dundee, United Kingdom

    Paul Birch

  2. Cell and Molecular Sciences,The James Hutton Institute, Dundee, United Kingdom

    John T. Jones

  3. Cell and Molecular Sciences, The James Hutton Institute, Dundee, United Kingdom

    Jorunn I.B. Bos

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Tomé, D.F.A., Steinbrenner, J., Beynon, J.L. (2014). A Growth Quantification Assay for Hyaloperonospora arabidopsidis Isolates in Arabidopsis thaliana . In: Birch, P., Jones, J., Bos, J. (eds) Plant-Pathogen Interactions. Methods in Molecular Biology, vol 1127. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-986-4_12

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  • DOI: https://doi.org/10.1007/978-1-62703-986-4_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-985-7

  • Online ISBN: 978-1-62703-986-4

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