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Super-Resolution Microscopy: Principles, Techniques, and Applications

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Super-Resolution Microscopy Techniques in the Neurosciences

Part of the book series: Neuromethods ((NM,volume 86))

Abstract

Diffraction sets a physical limit for the theoretically achievable resolution; however, it is possible to circumvent this barrier. That’s what microscopists have been doing in recent years and in many ways at once, starting the era of super-resolution in light microscopy. High-resolution approaches come in various flavors, and each has specific advantages or disadvantages. For example, near-field techniques avoid the problems associated with the propagation of light by getting very close to the specimen. In the far-field, the strategies include increasing the light collecting capability, sharpening the point spread function or high-precision localization of individual fluorescent molecules. In this chapter, the major super-resolution approaches are introduced briefly, together with their pros and cons, and exemplar biological applications.

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  1. Department of Neuro- and Sensory Physiology, University of Göttingen Medical Center, Göttingen, Germany

    Sinem K. Saka

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  1. Department of Neuro- and Sensory Physiology,University of Göttingen Medical Center, Göttingen, Germany

    Eugenio F. Fornasiero

  2. Department of Neuro- and Sensory Physiology,University of Göttingen Medical Center, Göttingen, Germany

    Silvio O. Rizzoli

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Saka, S.K. (2014). Super-Resolution Microscopy: Principles, Techniques, and Applications. In: Fornasiero, E., Rizzoli, S. (eds) Super-Resolution Microscopy Techniques in the Neurosciences. Neuromethods, vol 86. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-983-3_2

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