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RecTEPsy-Mediated Recombineering in Pseudomonas syringae

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Gene Correction

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1114))

Abstract

A recently developed Pseudomonas syringae recombineering system simplifies the procedure for installing specific mutations at a chosen genomic locus. The procedure involves transforming P. syringae cells expressing recombineering functions with a PCR product that contains desired changes flanked by sequences homologous to a target location. Cells transformed with the substrate undergo homologous recombination between the genomic DNA and the recombineering substrate. The recombinants are found by selection for traits carried by the recombineering substrate, usually antibiotic resistance.

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Acknowledgments

Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Plant–Microbe Interactions Research Unit, Department of Agriculture, Agricultural Research Service, Ithaca, NY, USA

    Bryan Swingle

Authors
  1. Bryan Swingle
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Editors and Affiliations

  1. Georgia Institute of Technology, Atlanta, Georgia, USA

    Francesca Storici

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Swingle, B. (2014). RecTEPsy-Mediated Recombineering in Pseudomonas syringae . In: Storici, F. (eds) Gene Correction. Methods in Molecular Biology, vol 1114. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-761-7_1

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  • DOI: https://doi.org/10.1007/978-1-62703-761-7_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-760-0

  • Online ISBN: 978-1-62703-761-7

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