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DNA Profiling and Characterization of Animal Cell Lines

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Animal Cell Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1104))

Abstract

The history of the culture of animal cell lines is littered with published and much unpublished experience with cell lines that have become switched, mislabelled, or cross-contaminated during laboratory handling. To deliver valid and good quality research and to avoid waste of time and resources on such rogue lines, it is vital to perform some kind of qualification for the provenance of cell lines used in research and particularly in the development of biomedical products. DNA profiling provides a valuable tool to compare different sources of the same cells and, where original material or tissue is available, to confirm the correct identity of a cell line. This chapter provides a review of some of the most useful techniques to test the identity of cells in the cell culture laboratory and gives methods which have been used in the authentication of cell lines.

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Author information

Authors and Affiliations

  1. National Institute for Biological Standards and Control, South Mimms, UK

    Glyn N. Stacey, Ed Byrne & J. Ross Hawkins

Authors
  1. Glyn N. Stacey
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  2. Ed Byrne
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  3. J. Ross Hawkins
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Editors and Affiliations

  1. Bioprozess- und Biosystemtechnik, Technische Universität Hamburg-Harburg, Hamburg, Germany

    Ralf Pörtner

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Stacey, G.N., Byrne, E., Hawkins, J.R. (2014). DNA Profiling and Characterization of Animal Cell Lines. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 1104. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-733-4_5

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  • DOI: https://doi.org/10.1007/978-1-62703-733-4_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-732-7

  • Online ISBN: 978-1-62703-733-4

  • eBook Packages: Springer Protocols

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