Abstract
Immunohistochemistry is an essential tool for clinical and translational research laboratories. It is mostly used as a qualitative measure of morphology and cell types within tissue. We have developed a high-resolution multispectral imaging method to expand the uses of immunohistochemistry by making it quantitative. In this chapter we describe the technology, both hardware and software, that we use for this method and give examples of applications.
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Acknowledgment
This work was supported by the US National Institute of Health National Cancer Institute grant R01 CA127947-03 and the US Department of Defense Era of Hope grant W81XWH-12-1-0366.
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Blenman, K.R.M., Lee, P.P. (2014). Quantitative and Spatial Image Analysis of Tumor and Draining Lymph Nodes Using Immunohistochemistry and High-Resolution Multispectral Imaging to Predict Metastasis. In: Thurin, M., Marincola, F. (eds) Molecular Diagnostics for Melanoma. Methods in Molecular Biology, vol 1102. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-727-3_32
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DOI: https://doi.org/10.1007/978-1-62703-727-3_32
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