Abstract
Cellular population dynamics are routinely monitored across many diverse fields for a variety of purposes. In general, these dynamics are assayed either through the direct counting of cellular aliquots followed by extrapolation to the total population size, or through the monitoring of signal intensity from any number of externally stimulated reporter proteins. While both viable methods, here we describe a novel technique that allows for the automated, non-destructive tracking of cellular population dynamics in real-time. This method, which relies on the detection of a continuous bioluminescent signal produced through expression of the bacterial luciferase gene cassette, provides a low cost, low time-intensive means for generating additional data compared to alternative methods.
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References
Soto A et al (1995) The E-SCREEN assay as a tool to identify estrogens: An update on estrogenic environmental pollutants. Environ Health Perspect 103:113–122
Yang X et al (2011) Novel membrane-associated androgen receptor splice variant potentiates proliferative and survival responses in prostate cancer cells. J Biol Chem 286:36152–36160
Rudin M, Weissleder R (2003) Molecular imaging in drug discovery and development. Nat Rev Drug Discov 2:123–131
Houck K, Kavlock R (2008) Understanding mechanisms of toxicity: Insights from drug discovery research. Toxicol Appl Pharmacol 227:163–178
Contag C, Bachmann M (2002) Advances in in vivo bioluminescence imaging of gene expression. Annu Rev Biomed Eng 4:235–260
Close D et al (2011) Light without substrate amendment: the bacterial luciferase gene cassette as a mammalian bioreporter. Proc SPIE 8029: 80291F-1-80290F-10
Close D et al (2011) Comparison of human optimized bacterial luciferase, firefly luciferase, and green fluorescent protein for continuous imaging of cell culture and animal models. J Biomed Opt 16:047003
Tsien R (1998) The green fluorescent protein. Annu Rev Biochem 67:509–544
Meighen E (1991) Molecular biology of bacterial bioluminescence. Microbiol Rev 55:123–142
Close D et al (2010) Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell line. PLoS ONE 5:e12441
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Close, D., Xu, T., Ripp, S., Sayler, G. (2014). Real-Time Bioluminescent Tracking of Cellular Population Dynamics. In: Badr, C. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 1098. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-718-1_9
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DOI: https://doi.org/10.1007/978-1-62703-718-1_9
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-717-4
Online ISBN: 978-1-62703-718-1
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