Abstract
Denaturing gradient gel electrophoresis (DGGE) is a culture-independent fingerprinting technique that allows for rapid comparative analysis of changes to microbial communities. 16S rRNA genes amplified from environmental samples can be separated based on their melting behavior in a denaturing gradient of urea and formamide. A fingerprint of the microbial community is generated with each band on the gel assumed to correspond to a different bacterial species. Community dynamics can then be assessed through statistical analysis of DGGE profiles and the sequencing of excised bands.
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Thompson, C.L. (2014). Analysis of Community Dynamics in Environmental Samples Using Denaturing Gradient Gel Electrophoresis. In: Paulsen, I., Holmes, A. (eds) Environmental Microbiology. Methods in Molecular Biology, vol 1096. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-712-9_4
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DOI: https://doi.org/10.1007/978-1-62703-712-9_4
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-712-9
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