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FISH Methods in Cytogenetic Studies

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Functional Analysis of DNA and Chromatin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1094))

Abstract

This chapter describes the various methods derived from the protocol of standard fluorescent in situ hybridization (FISH) that are used in human, animal, plant, and microbial studies. These powerful techniques allow us to detect and physically map on interphase nuclei, chromatin fibers, or metaphase chromosomes probes derived from single-copy genes to repetitive DNA sequences. Other variants of the technique enable the co-localization of genes and the overall comparison of the genome among individuals of the same species or of different taxa. A further variant detects and localizes bacteria on tissues and cells. Overall, this offers a remarkable multiplicity of possible applications ranging from strict physical mapping, to clinical and evolutionary studies, making it a powerful and informative complement to other molecular, functional, or genomic approaches.

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Acknowledgments

We thank Dr. P.L. Mason (University of Glasgow) for revising the manuscript. This work has been supported by the Spanish grants CGL2009-08380/BOS and CGL2012-35007/BOS.

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Authors and Affiliations

  1. Departamento de Biología (Genética), Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain

    Miguel Pita, Paloma Martínez-Rodríguez, Begoña Fernández-Calvín & José L. Bella

  2. Departamento de Biotecnología, ETS Ingenieros Agrónomos, Universidad Politécnica de Madrid, Madrid, Spain

    Juan Orellana

  3. Molecular Cytogenetics Laboratory, MD Anderson Cancer Center, Madrid, Spain

    Ángel Martínez-Ramírez

Authors
  1. Miguel Pita
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  2. Juan Orellana
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  3. Paloma Martínez-Rodríguez
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  4. Ángel Martínez-Ramírez
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  5. Begoña Fernández-Calvín
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  6. José L. Bella
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Editor information

Editors and Affiliations

  1. Department of Biology, Autonomous University of Madrid Faculty of Sciences, Madrid, Spain

    Juan C. Stockert

  2. Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain

    Jesús Espada

  3. Department of Biology, Autonomous University of Madrid Faculty of Sciences, Madrid, Spain

    Alfonso Blázquez-Castro

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Pita, M., Orellana, J., Martínez-Rodríguez, P., Martínez-Ramírez, Á., Fernández-Calvín, B., Bella, J.L. (2014). FISH Methods in Cytogenetic Studies. In: Stockert, J., Espada, J., Blázquez-Castro, A. (eds) Functional Analysis of DNA and Chromatin. Methods in Molecular Biology, vol 1094. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-706-8_10

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  • DOI: https://doi.org/10.1007/978-1-62703-706-8_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-705-1

  • Online ISBN: 978-1-62703-706-8

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