Abstract
Embryonic stem (ES) cells are derived from the inner cell masses of preimplantation embryos. ES cells are pluripotent cells with the capacity for long-term propagation and broad differentiation plasticity. These cells have an exceptional functional feature in that they can differentiate into all tissues and organs, including germ cells. Established ES cell lines have been generated in mouse, human, and nonhuman primate but derivation of ES cells in farm animals has been problematic. Several ES-like cell lines from farm animals have been reported to exhibit properties of pluripotency in vitro. However, only a few of them morphologically resemble ES cells, or express markers that are associated with established ES cell lines from mouse and humans. Methods for derivation, propagation, and differentiation of ES cells from domestic animals have not been fully established. In this chapter, we describe methods for isolation of goat ES (gES) cell lines from in vivo-derived blastocysts and characterization of markers indicative of pluripotency. In addition, we outline differentiation of gES cells into all three germ layers in vivo by forming teratomas as a hallmark of pluripotency.
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References
Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154–156
Thomson JA, Kalishman J, Golos TG et al (1995) Isolation of a primate embryonic stem cell line. Proc Natl Acad Sci U S A 92:7844–7848
Thomson JA, Itskovitz-Eldor J, Shapiro SS et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1147
Stice SL, Strelchenko NS, Keefer CL et al (1996) Pluripotent bovine embryonic cell lines direct embryonic development following nuclear transfer. Biol Reprod 54:100–110
Mitalipova M, Beyhan Z, First NL (2001) Pluripotency of bovine embryonic cell line derived from precompacting embryos. Cloning 3:59–67
Wang L, Duan E, Sung LY et al (2005) Generation and characterization of pluripotent stem cells from cloned bovine embryos. Biol Reprod 73:149–155
Cibelli JB, Stice SL, Golueke PL et al (1998) Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280:1256–1258
Van Eijk MJT, van Rooijen MA, Modina S et al (1999) Molecular cloning, genetic mapping, and developmental expression of bovine POU5F1. Biol Reprod 60:1093–1103
Kirchhof N, Carnwath JW, Lemme E et al (2000) Expression pattern of Oct-4 in preimplantation embryos of different species. Biol Reprod 63:1698–1705
He H, Pant D, Schiffmacher A et al (2006) Developmental expression of pluripotency determining factors in caprine embryos: novel pattern of NANOG protein localization in the nucleolus. Mol Reprod Dev 73:1512–1522
Meinecke-Tillmann S, Meinecke B (1996) Isolation of ES-like cell lines from ovine and caprine pre-implantation embryos. J Anim Breed Genet 113:413–426
He S, Plant D, Bischoff S, Gavin W, Melican D, Keefer CL (2005) Expression of pluripotency-determining factors Oct-4 and Nanog in preimplantation goat embryos. Reprod Fert Dev 17:203
Behboodi E, Bondareva A, Begin I et al (2011) Establishment of goat embryonic stem cells from in vivo produced blastocyst-stage embryos. Mol Reprod Dev 78:202–211
Garg S, Dutta R, Malakar D et al (2012) Cardiomyocytes rhythmically beating generated from goat embryonic stem cell. Theriogenology 77:829–839
De AK, Malakar D, Akshey YS et al (2011) Isolation and characterization of embryonic stem cell-like cells from in vitro produced goat (Capra hircus) embryos. Anim Biotechnol 22:181–196
Baldassarre H, Karatzas CN (2004) Advanced assisted reproduction technologies (ART) in goats. Anim Reprod Sci 82–83:255–266
Melican D, Gavin D (2008) Non-surgical embryo retrieval utilizing repeat superovulation and surgical embryo transfer in transgenic dairy goats. Theriogenology 69:197–203
Prokhorova TA, Harkness LM, Frandsen U et al (2009) Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of matrigel. Stem Cell Dev 18:47–54
Martin-Ibanez R, Unger C, Stromberg A et al (2008) Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor. Hum Reprod 23:2744–2754
Acknowledgements
This work was supported by Discovery Grant 385837-2010 and a Discovery Accelerator Supplement from the National Science and Engineering Research Council (NSERC) of Canada.
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Behboodi, E., Lam, L., Gavin, W.G., Bondareva, A., Dobrinski, I. (2013). Goat Embryonic Stem-Like Cell Derivation and Characterization. In: Alberio, R. (eds) Epiblast Stem Cells. Methods in Molecular Biology, vol 1074. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-628-3_5
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DOI: https://doi.org/10.1007/978-1-62703-628-3_5
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Publisher Name: Humana Press, Totowa, NJ
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