Abstract
The principle of commonly used methods to create mutations in the nematode Caenorhabditis elegans (C. elegans) is straightforward. In general, worms are exposed to a dose of mutagen resulting in DNA damages and mutations. Screening the progeny of the mutagenized animals for a certain phenotype is the regular forward genetic approach in C. elegans. A mutant selected from such a population is stabilized to recover a pure homozygous strain. In this chapter, we categorize the protocol into mutagenesis, phenotype screen, and outcross and provide time-tested procedures for their implementation to create long-lived worm mutants.
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Acknowledgements
We are indebted to A. Brown for editorial assistance. D.W. was supported by the National Natural Science Foundation of China (No. 81172698). Y.D. and M.C. are supported by Creative Inquiry fund of Clemson University and Yamada Research Grant. Y.D. was supported by grant from AFAR.
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Wang, D., Cao, M., Dinh, J., Dong, Y. (2013). Methods for Creating Mutations in C. elegans That Extend Lifespan. In: Tollefsbol, T. (eds) Biological Aging. Methods in Molecular Biology, vol 1048. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-556-9_6
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DOI: https://doi.org/10.1007/978-1-62703-556-9_6
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