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Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in Low-Water Media

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Immobilization of Enzymes and Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1051))

Abstract

A simple immobilization method for Candida rugosa lipase on superparamagnetic Fe3O4 nanoparticles is described. The Fe3O4 nanoparticles were coated with PEI and Candida rugosa lipase was adsorbed on these particles via electrostatic interactions. The immobilization resulted in marginal simultaneous purification. However, the immobilized preparation showed 110× higher transesterification activity in low-water media. It was also efficient in kinetic resolution of (±)-1-phenylethanol with eep of 99 % and E = 412 within 24 h.

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Acknowledgments

Financial support from the Department of Science and Technology (DST-SERB) [Grant No.: SR/SO/BB-68/2010], the Department of Biotechnology (DBT) [Grant Number: BT/PR14158/NNT/28/484/2010], and Council for Scientific and Industrial Research (CSIR), all Government of India organizations, is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Indian Institute of Technology Delhi, New Delhi, India

    Joyeeta Mukherjee & Munishwar Nath Gupta

  2. Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA

    Kusum Solanki

Authors
  1. Joyeeta Mukherjee
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  2. Kusum Solanki
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  3. Munishwar Nath Gupta
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Editors and Affiliations

  1. Instituto de Catlisis y Petroleoquimica, CSIC, Madrid, Spain

    Jose M. Guisan

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Mukherjee, J., Solanki, K., Gupta, M.N. (2013). Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in Low-Water Media. In: Guisan, J. (eds) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 1051. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-550-7_8

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  • DOI: https://doi.org/10.1007/978-1-62703-550-7_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-549-1

  • Online ISBN: 978-1-62703-550-7

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