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Cardiac Wound Healing Post-myocardial Infarction: A Novel Method to Target Extracellular Matrix Remodeling in the Left Ventricle

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Wound Regeneration and Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1037))

Abstract

Myocardial infarction (MI) is a leading cause of death worldwide. Permanent ligation of the left anterior descending coronary artery (LAD) is a commonly used surgical model to study post-MI effects in mice. LAD occlusion induces a robust wound healing response that includes extracellular matrix (ECM) remodeling. This chapter provides a detailed guide on the surgical procedure to permanently ligate the LAD. Additionally, we describe a prototype method to enrich cardiac tissue for ECM, which allows one to focus on ECM remodeling in the left ventricle following surgically induced MI in mice.

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Acknowledgements

We acknowledge funding from AHA (09POST2150178) to RZ, NCCAM (1K99AT006704-01) to GH, and NHLBI HHSN 268201000036C (N01-HV-00244) for the UTHSCSA Cardiovascular Proteomics Center and R01 HL-075360, the Max and Minnie Tomerlin Voelcker Fund, and the Veteran’s Administration (Merit) to MLL.

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Authors and Affiliations

  1. Division of Geriatrics, Gerontology and Palliative Medicine, Department of Medicine, Barshop Institute of Longevity and Aging Studies, The University of Texas Health Science Center, San Antonio, TX, USA

    Rogelio Zamilpa, Jianhua Zhang, Ying Ann Chiao, Lisandra E. de Castro Brás, Ganesh V. Halade, Yonggang Ma & Merry L. Lindsey

  2. Department of Biochemistry, Barshop Institute of Longevity and Aging Studies, The University of Texas Health Science Center, San Antonio, TX, USA

    Ying Ann Chiao

  3. Department of Laboratory Animal Resources, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA

    Sander O. Hacker

Authors
  1. Rogelio Zamilpa
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  2. Jianhua Zhang
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  3. Ying Ann Chiao
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  4. Lisandra E. de Castro Brás
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  5. Ganesh V. Halade
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  6. Yonggang Ma
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  7. Sander O. Hacker
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  8. Merry L. Lindsey
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Editor information

Editors and Affiliations

  1. Virginia Tech Carilion Research Institut, Roanoke, Virginia, USA

    Robert G. Gourdie

  2. School of Pharmacy, MCPHS University, Worcester, Massachusetts, USA

    Tereance A. Myers

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Zamilpa, R. et al. (2013). Cardiac Wound Healing Post-myocardial Infarction: A Novel Method to Target Extracellular Matrix Remodeling in the Left Ventricle. In: Gourdie, R., Myers, T. (eds) Wound Regeneration and Repair. Methods in Molecular Biology, vol 1037. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-505-7_18

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  • DOI: https://doi.org/10.1007/978-1-62703-505-7_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-504-0

  • Online ISBN: 978-1-62703-505-7

  • eBook Packages: Springer Protocols

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