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Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE

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Mouse Models of Allergic Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1032))

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Abstract

Immunoglobulins (Igs) are a critical component of the adaptive immune system of both man and mouse. The ability to detect and characterize Igs is an invaluable technique for immunology in either a research or a clinical setting. The advent of enzyme-linked immunosorbent assays (ELISAs) and monoclonal antibody technology has proven instrumental for advancing the science of Ig biology. IgE is of interest as it is the primary Ig responsible for allergic reactions ranging from allergic rhinitis to anaphylaxis. Here, we describe the history behind the IgE discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is followed by our ELISA protocol for mouse IgE detection.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA

    Jamie L. Sturgill & Daniel H. Conrad

Authors
  1. Jamie L. Sturgill
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  2. Daniel H. Conrad
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Editor information

Editors and Affiliations

  1. Dept. of Biomedical Sciences & Pathobiol, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    Irving C. Allen

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Sturgill, J.L., Conrad, D.H. (2013). Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE. In: Allen, I. (eds) Mouse Models of Allergic Disease. Methods in Molecular Biology, vol 1032. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-496-8_9

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  • DOI: https://doi.org/10.1007/978-1-62703-496-8_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-495-1

  • Online ISBN: 978-1-62703-496-8

  • eBook Packages: Springer Protocols

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