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Human Leukocyte Antigen (HLA) Typing by DNA Sequencing

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Transplantation Immunology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1034))

Abstract

DNA sequencing is a powerful technique for identifying allelic variation within the human leukocyte antigen (HLA) genes. Sequencing is usually focused on the most polymorphic exons of the class I (HLA-A, -B, -C) and class II (HLA-DR, -DQ, and -DP) genes. These exons encode the antigen recognition site, the region of the HLA molecule that binds peptides and interacts with the T cell receptor for antigen and natural killer cell immunoglobulin-like receptors (KIR). Sanger sequencing of amplified DNA from each HLA gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a reference database.

Ana Lazaro and Bin Tu have contributed equally to first authorship.

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Author information

Authors and Affiliations

  1. CW Bill Young Marrow Donor Recruitment and Research Program, Department of Pediatrics, Georgetown University Medical Center, Rockville, MD, USA

    Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam & Jennifer Ng

  2. CW Bill Young Marrow Donor Recruitment and Research Program, Department of Oncology, Georgetown University Medical Center, Washington, DC, USA

    Carolyn Katovich Hurley

Authors
  1. Ana Lazaro
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  2. Bin Tu
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  3. Ruyan Yang
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  4. Yi Xiao
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  5. Kanthi Kariyawasam
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  6. Jennifer Ng
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  7. Carolyn Katovich Hurley
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Editor information

Editors and Affiliations

  1. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Andrea A. Zachary

  2. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Mary S. Leffell

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Lazaro, A. et al. (2013). Human Leukocyte Antigen (HLA) Typing by DNA Sequencing. In: Zachary, A., Leffell, M. (eds) Transplantation Immunology. Methods in Molecular Biology, vol 1034. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-493-7_9

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  • DOI: https://doi.org/10.1007/978-1-62703-493-7_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-492-0

  • Online ISBN: 978-1-62703-493-7

  • eBook Packages: Springer Protocols

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