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Application of Microfluidics to Study Stem Cell Dynamics

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Emerging Trends in Cell and Gene Therapy

Abstract

Stem cell fate is directed by a complex chemical and mechanical microenvironment composed of secreted factors, extracellular matrix, and direct interactions with other cells. These signals ultimately control stem cell renewal and lineage fate in a developmental context. It may be possible to dissect the role of specific signaling pathways by precise control of microenvironment. However, traditional flask cell culture methods are unable to control microenvironment at microscale. Microfluidic platforms have the potential of mimicking the signals that direct stem cell fate by precise control of the chemical and mechanical milieu of cells at microscale. Furthermore, so called “lab-on-a-chip” technologies can increase research throughput by cost-effect automation of multiple parallel microscale cultures. This chapter will reveal how microfluidics and lab-on-a-chip technologies can be applied to the study of stem cell dynamics.

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Authors and Affiliations

  1. Faculty of Engineering, Graduate School of Biomedical Engineering, The University of New South Wales, Samuels Building, Sydney, NSW, 2052, Australia

    Huaying Chen

  2. School of Materials Science and Engineering, Shandong University, Ji’nan, China

    Huaying Chen

  3. Graduate School of Biomedical Engineering, The University of New South Wales, Samuels Building, Sydney, NSW, 2052, Australia

    Robert E. Nordon

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  1. Huaying Chen
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  2. Robert E. Nordon
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Correspondence to Robert E. Nordon .

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  1. Pharmaceutical Sciences, Douglas Hall 206, Chicago State University, S. King Drive 9501, Chicago, 60628, Illinois, USA

    Michael K. Danquah

  2. Health Science Center, Dept. Pharmaceutical &, University of Tennessee, S. Manassas 19, Memphis, 38103, Tennessee, USA

    Ram I. Mahato

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Chen, H., Nordon, R.E. (2013). Application of Microfluidics to Study Stem Cell Dynamics. In: Danquah, M., Mahato, R. (eds) Emerging Trends in Cell and Gene Therapy. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-417-3_19

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