Abstract
Plant phospholipids and glycolipids can be analyzed by direct infusion electrospray ionization triple-quadrupole mass spectrometry. A biological extract is introduced in solvent by continuous infusion into the mass spectrometer’s electrospray ionization source, where ions are produced from the lipids. For analysis of membrane lipids, a series of precursor and neutral loss scans, each specific for lipids containing a common head group, are obtained sequentially. The mass spectral data are processed and combined, using the Web application LipidomeDB Data Calculation Environment, to create a lipid profile.
Sunitha Shiva and Hieu Sy Vu have contributed equally.
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Acknowledgments
The development of plant lipidomics methodology was supported by the National Science Foundation (MCB 0455318 and MCB 0920663) to R.W., Xuemin Wang, Jyoti Shah, Todd Williams, and Gary Gadbury. We are very grateful to these, other colleagues, and lab members who have contributed to plant lipidomics development. Contribution no. 11-300-B from the Kansas Agricultural Experiment Station.
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Shiva, S. et al. (2013). Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry. In: Munnik, T., Heilmann, I. (eds) Plant Lipid Signaling Protocols. Methods in Molecular Biology, vol 1009. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-62703-401-2_9
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DOI: https://doi.org/10.1007/978-1-62703-401-2_9
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