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Labeled Stem Cells as Disease Models and in Drug Discovery

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Pluripotent Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 997))

Abstract

Human pluripotent stem cells provide unique possibilities for in vitro studies of human cells in basic research, disease modeling as well as in industrial applications. By introducing relevant genome engineering technology, and thereby creating, for example, reporter cell lines, one will facilitate and improve safety pharmacology, toxicity testing, and can help the scientists to better understand pathological processes in humans. This review discusses how the merger of these two fields, human pluripotent stem cells and genome engineering, form extremely powerful tools and how they have been implemented already within the scientific community. In sharp contrast to immortalized human cell lines, which are both easy to expand and very simple to transfect, the genetically modified pluripotent stem cell line can be directed to a specific cell lineage and provide the user with highly relevant information. We highlight some of the challenges the field had to solve and how new technology advancements has removed the early bottlenecks.

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Acknowledgments

The authors are grateful to Drs. Bhaskar Thyagarajan, Jon Chesnut, and Mahendra Rao for fruitful collaboration and for providing the tools for the Jump In technology. The authors would also like to thank Drs. Charles Hanson, Ulf Stenevi, and Thorir Hardarson for the providing the picture with human corna, and Tina Nilsson, Jenny Johannisson, Dorra El Hajjam, Ingrid Rydström, and Jenny Lindqvist for the execution and characterization of the reporter lines shown in the figures of this chapter.

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Authors and Affiliations

  1. Cellectis Stem Cells, Cellartis AB, Göteborg, Sweden

    Catharina Ellerström, Raimund Strehl & Johan Hyllner

Authors
  1. Catharina Ellerström
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  2. Raimund Strehl
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  3. Johan Hyllner
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Editors and Affiliations

  1. , Primary and Stem Cell Systems, Life Technologies, Van Allen Way 5781, Carlsbad, 92008, California, USA

    Uma Lakshmipathy

  2. , Primary and Stem Cell Systems, Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA

    Mohan C Vemuri

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Ellerström, C., Strehl, R., Hyllner, J. (2013). Labeled Stem Cells as Disease Models and in Drug Discovery. In: Lakshmipathy, U., Vemuri, M. (eds) Pluripotent Stem Cells. Methods in Molecular Biology, vol 997. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-348-0_19

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  • DOI: https://doi.org/10.1007/978-1-62703-348-0_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-347-3

  • Online ISBN: 978-1-62703-348-0

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