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Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy

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Plant Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 990))

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Abstract

Transmission electron microscopy (TEM) of thin sections of meiocytes embedded in plastic resin allows analysis of structures in meiotic nuclei, such as the synaptonemal complex, at very high resolution while preserving the spatial organization of chromosomes.

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References

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Author information

Authors and Affiliations

  1. Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia

    Ljudmilla Timofejeva

Authors
  1. Ljudmilla Timofejeva
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Editor information

Editors and Affiliations

  1. , Dept. of Plant Breeding & Genetics, Cornell University, Bradfield Hall 401, Ithaca, 14853, New York, USA

    Wojciech P. Pawlowski

  2. , Centre de Versailles-Grignon, Inst National de la Recherche Agronomiqu, Route de St-Cyr (RD10), Versailles, F-78000, France

    Mathilde Grelon

  3. School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom

    Susan Armstrong

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Timofejeva, L. (2013). Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy. In: Pawlowski, W., Grelon, M., Armstrong, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 990. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-333-6_17

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  • DOI: https://doi.org/10.1007/978-1-62703-333-6_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-332-9

  • Online ISBN: 978-1-62703-333-6

  • eBook Packages: Springer Protocols

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