Abstract
Identification and verification of novel transcription factor interactions is an inherent step in the discovery of molecular mechanisms driving gene transcription and regulation. Co-immunoprecipitation and GST-pull down are often key techniques in the verification process. Despite wide applicability, their use may sometimes be restricted. We provide a detailed protocol for an intracellular immunofluorescence technique that may be used as an alternative or complimentary study for transcription factor interaction verification.
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Acknowledgments
This work was supported by European Community’s Sixth FrameÂwork Programme contract HeartRepair LSHM-CT-2005-018630.
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Boogerd, C.J.J., Christoffels, V.M., Barnett, P. (2013). Nuclear Recruitment Assay as a Tool to Validate Transcription Factor Interactions in Mammalian Cells. In: Bina, M. (eds) Gene Regulation. Methods in Molecular Biology, vol 977. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-284-1_19
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DOI: https://doi.org/10.1007/978-1-62703-284-1_19
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-283-4
Online ISBN: 978-1-62703-284-1
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