Abstract
Dopamine is the main catecholamine found in the retina of most species, being synthesized from the l-amino acid tyrosine. Its effects are mediated by G protein coupled receptors subfamilies that are commonly coupled to adenylyl cyclase in opposite manners. There is evidence that this amine works as a developmental signal in the embryonic retina and several distinct roles have been attributed to dopamine in the retina such as proliferation, synaptogenesis, neuroprotection, increased signal transmission in cone, gap junction modulation, neuronal–pigmented epithelium–glial communication, and neuron–glia interaction. Here we describe methods that have been used in the study of the dopaminergic function in the retina in the last 40 years. We emphasize the approaches used in the studies on the development of the avian and rodent retina. The dopaminergic system is one of the first phenotypes to appear in the developing vertebrate retina.
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References
Witkovsky P, Dearry A (1992) Functional roles of dopamine in the vertebrate retina. Prog Retinal Res 11:247–292
Reis RAM, Ventura ALV, Kubrusly RC, de Mello MC, de Mello FG (2007) Dopaminergic signaling in the developing retina. Brain Res Rev 54:181–188
de Mello FG (1978) The ontogeny of dopamine-dependent increase of adenosine 3′,5′-cyclic monophosphate in the chick retina. J Neurochem 31:1049–1053
Gardino PF, dos Santos RM, Hokoc JN (1993) Histogenesis and topographical distribution of tyrosine hydroxylase immunoreactive amacrine cells in the developing chick retina. Brain Res Dev Brain Res 72:226–236
Ventura ALM, Klein WL, de Mello FG (1984) Differential ontogenesis of D1 and D2 dopaminergic receptors in the chick embryo retina. Brain Res 314:217–223
Kubrusly RCC, Guimarães MPZ, Vieira APB, Hokoç JN, Casarini DE, de Mello MC, de Mello FG (2003) L-DOPA supply to the neuro retina activates dopaminergic communication at the early stages of embryonic development. J Neurochem 86:45–54
Kubrusly RC, Panizzutti R, Gardino PF, Stutz B, Reis RA, Ventura AL, de Mello MC, de Mello FG (2008) Expression of functional dopaminergic phenotype in purified cultured Müller cells from vertebrate retina. Neurochem Int 53:63–70
de Melo Reis RA, Ventura ALV, Schitine CS, de Mello MC, de Mello FG (2008) Müller glia as an active compartment modulating nervous activity in the vertebrate retina: neurotransmitters and trophic factors. Neurochem Res 33:1466–1474
Soares HC, Reis RA, De Mello FG, Ventura AL, Kurtenbach E (2000) Differential expression of D(1A) and D(1B) dopamine receptor mRNAs in the developing avian retina. J Neurochem 75:1071–1075
Kubrusly RC, da Cunha MC, Reis RA, Soares H, Ventura AL, Kurtenbach E, de Mello MC, de Mello FG (2005) Expression of functional receptors and transmitter enzymes in cultured Müller cells. Brain Res 1038:141–149
Ventura ALM, de Mello FG (1990) D1 dopamine receptors in neurite regions of embryonic and differentiated retina are highly coupled to adenylyl cyclase in the embryonic but not in the mature tissue. Brain Res 530:301–308
Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York
Reis RA, Cabral da Silva MC, Loureiro dos Santos NE, Bampton E, Taylor JS, de Mello FG, Linden R (2002) Sympathetic neuronal survival induced by retinal trophic factors. J Neurobiol 50:13–23
Kubrusly RC, Ventura AL, Reis RA et al (2007) Norepinephrine acts as D1-dopaminergic agonist in the embryonic avian retina: late expression of β1-adrenergic receptor shifts norepinephrine specificity in the adult tissue. Neurochem Int 50:211–218
Guimarães MZP, Hokoç JN, Duvoisin R et al (2001) Dopaminergic retinal cell differentiation in culture: modulation by forskolin and dopamine. Eur J Neurosci 13:1931–1937
Borba JC, Henze IP, Silveira MS et al (2005) Pituitary adenylate cyclase-activating polypeptide (PACAP) can act as determinant of the tyrosine hydroxylase phenotype of dopaminergic cells during retina development. Brain Res Dev Brain Res 156:193–201
Dos Santos RM, Gardino PF (1998) Differential distribution of a second type of tyrosine hydroxylase immunoreactive amacrine cell in the chick retina. J Neurocytol 27:33–43
Taveira da Silva R, Hokoç JN, de Mello FG et al (2009) Differential immunodetection of L-DOPA decarboxylase and tyrosine hydroxylase in the vertebrate retina. Int J Dev Neurosci 27:469–476
De Mello MC, Pinheiro MC, de Mello FG (1996) Transient expression of an atypical D1-like dopamine receptor system during avian retina differentiation. Braz J Med Biol Res 29:1035–1044
de Mello MC, Ventura ALM, Paes de Carvalho R, Klein WL, de Mello FG (1982) Regulation of dopamine and adenosine-dependent adenylate cyclase systems of chick embryo retina cells in culture. Proc Natl Acad Sci USA 79:5708–5712
Arita DY, Di Marco GS, Schor N, Casarini DE (2002) Purification and characterization of the active form of tyrosine hydroxylase from mesangial cells in culture. J Cell Biochem 87:58–64
Lankford KL, De Mello FG, Klein WL (1988) D1-type dopamine receptors inhibit growth cone motility in cultured retina neurons: evidence that neurotransmitters act as morphogenic growth regulators in the developing central nervous system. Proc Natl Acad Sci USA 85:2839–2843
Varella MH, de Mello FG, Linden R (1999) Evidence for an antiapoptotic role of dopamine in developing retinal tissue. J Neurochem 73:485–492
Tibber MS, Whitmore AV, Jeffery G (2006) Cell division and cleavage orientation in the developing retina are regulated by L-DOPA. J Comp Neurol 496:369–381
Do Nascimento JLM, Kubrusly RCC, Reis RAM, De Mello MC, De Mello FG (1998) Atypical effect of dopamine in modulating the functional inhibition of NMDA receptors of cultured retina cells. Eur J Pharmacol 343:103–110
Castro NG, de Mello MC, de Mello FG, Aracava Y (1999) Direct inhibition of the N-methyl-d-aspartate receptor channel by dopamine and (+)-SKF38393. Br J Pharmacol 126:1847–1855
Wang HY, Undie AS, Friedman E (1995) Evidence for the coupling of Gq protein to D1-like dopamine sites in rat striatum: possible role in dopamine-mediated inositol phosphate formation. Mol Pharmacol 48:988–994
Reis RA, Kubrusly RC, de Mello MC, de Mello FG (1995) Transient coupling of NMDA receptor with ip3 production in cultured cells of the avian retina. Neurochem Int 26:375–380
Acknowledgements
This work was supported by grants from FAPERJ, CNPq, PROPPi-UFF and INCT-CNPq (INNT).
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Ventura, A.L.M., de Mello, F.G., de Melo Reis, R.A. (2013). Methods of Dopamine Research in Retina Cells. In: Kabbani, N. (eds) Dopamine. Methods in Molecular Biology, vol 964. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-251-3_3
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DOI: https://doi.org/10.1007/978-1-62703-251-3_3
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