Abstract
Major histocompatibility complex (MHC) class I peptide motifs are used on a regular basis to identify and predict MHC class I ligands and CD8+ T-cell epitopes. This approach is above all an invaluable tool for the identification of disease-associated epitopes. As a matter of fact, the vast majority of T-cell epitopes discovered during the past two decades was identified by means of epitope prediction. Here we describe the steps which are necessary to establish MHC class I peptide motifs and to compose a reliable scoring matrix for epitope prediction. As an example, a scoring matrix for the prediction of HLA-B*35-presented T-cell epitopes will be developed by examining the characteristics of 76 naturally presented HLA ligands.
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Trautwein, N., Stevanović, S. (2013). Establishing MHC Class I Peptide Motifs. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_13
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DOI: https://doi.org/10.1007/978-1-62703-218-6_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-217-9
Online ISBN: 978-1-62703-218-6
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