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Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun

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Biolistic DNA Delivery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 940))

Abstract

Biolistic DNA delivery is an approach to deliver plasmid to culture cells, plants, or animals. Plasmid DNA is usually transferred through bombardment of DNA-coated particles by highly pressurized gas in various kinds of delivery vehicles. The low pressure gene gun can deliver plasmid at lower pressure. Here, we describe methods of biolistic DNA delivery to mice using the low pressure gene gun.

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Acknowledgments

This work is supported by Grant NSC99-2323-B006-004 from National Science Council, Taiwan, Republic of China.

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Correspondence to Ming-Derg Lai .

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Yen, MC., Lai, MD. (2013). Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun. In: Sudowe, S., Reske-Kunz, A. (eds) Biolistic DNA Delivery. Methods in Molecular Biology, vol 940. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-110-3_14

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  • DOI: https://doi.org/10.1007/978-1-62703-110-3_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-109-7

  • Online ISBN: 978-1-62703-110-3

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