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Methods to Study the Effects of TRP Channel Drugs on Vascular Endothelial Cell Function

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TRP Channels in Drug Discovery

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

Transient receptor potential (TRP) channels are abundantly expressed in vascular endothelium where the channels play important functional roles. In this chapter, we describe some well-established approaches, from molecular detection to functional assays, to study the role of TRP channels in endothelial cells. Comprehensive step-by-step instructions and representative figures are provided on the following methods: (1) isolation and culture of vascular endothelial cells; (2) determination of the TRP channel interaction [co-immunoprecipitation, double immunofluorescence staining, chemical cross-linking, and Förster resonance energy transfer (FRET) detection]; (3) detection of TRP channel-mediated intracellular Ca2+ changes using fluorescence microscopy; (4) determination of the electrophysiological properties by whole-cell patch clamp and examination of TRP channel function in vascular tone control using isometric tension measurement.

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Acknowledgments

This work was supported by CUHK478011, CUHK479109, and CUHK478710 from the Hong Kong RGC, Strategic Investment Scheme C and Group Research Grant from Chinese University of Hong Kong.

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Authors and Affiliations

  1. Li Ka Shing Institute of Health Sciences, School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, China

    Yan Ma, Yung-Wui Tjong & Xiaoqiang Yao

Authors
  1. Yan Ma
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  2. Yung-Wui Tjong
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  3. Xiaoqiang Yao
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Corresponding author

Correspondence to Yan Ma .

Editor information

Editors and Affiliations

  1. Transfusion Services, Monmouth Medical Center, Long Branch, New Jersey, USA

    Arpad Szallasi

  2. Department of Physiology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary

    Tamás Bíró

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Ma, Y., Tjong, YW., Yao, X. (2012). Methods to Study the Effects of TRP Channel Drugs on Vascular Endothelial Cell Function. In: Szallasi, A., Bíró, T. (eds) TRP Channels in Drug Discovery. Methods in Pharmacology and Toxicology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-095-3_3

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  • DOI: https://doi.org/10.1007/978-1-62703-095-3_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-094-6

  • Online ISBN: 978-1-62703-095-3

  • eBook Packages: Springer Protocols

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