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Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells

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Calcium Signaling Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 937))

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Abstract

Measurement of calcium (Ca2+) fluorescence in conjunction with ionic currents is of particular importance in contractile cells, such as cardiac ventricular myocytes and vascular smooth muscle. The interplay between membrane potential and intracellular calcium ([Ca2+]i) is fundamental to the regulation of contractile function and cell signalling. Here the loading of cells either with an esterified fluorescence indicator prior to patch clamp recording, or dye loading via the patch pipette with “free” indicator, is described to allow simultaneous measurement of fluorescence and electrical signals.

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Acknowledgments

The author would like to thank Sean Brennan (University of Manchester) for his help with the recording shown in Fig. 1. The author would also like to thank Prof. John McCarron (University of Strathclyde) for allowing the recording of the ClCa current shown in Fig. 3 to be used in this chapter.

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Authors and Affiliations

  1. Department of Cardiovascular Sciences, Leicester Royal Infirmary, University of Leicester, Leicester, UK

    Richard D. Rainbow

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  1. Richard D. Rainbow
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Correspondence to Richard D. Rainbow .

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Editors and Affiliations

  1. , Department of Cardiovascular Sciences, Leicester Royal Infirmary, Infirmary Square, Leicester, LE1 5WW, Leicestershire, United Kingdom

    David G. Lambert

  2. , Department of Cardiovascular Sciences, Leicester Royal Infirmary, Infirmary Square, Leicester, LE1 5WW, Leicestershire, United Kingdom

    Richard D. Rainbow

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Rainbow, R.D. (2013). Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells. In: Lambert, D., Rainbow, R. (eds) Calcium Signaling Protocols. Methods in Molecular Biology, vol 937. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-086-1_8

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  • DOI: https://doi.org/10.1007/978-1-62703-086-1_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-085-4

  • Online ISBN: 978-1-62703-086-1

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