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How to Investigate Interactions Between Membrane Proteins and Ligands by Solid-State NMR

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Membrane Protein Structure and Dynamics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 914))

Abstract

Solid-state NMR is an established method for biophysical studies of membrane proteins within the lipid bilayers and an emerging technique for structural biology in general. In particular magic angle sample spinning has been found to be very useful for the investigation of large membrane proteins and their interaction with small molecules within the lipid bilayer. Using a number of examples, we illustrate and discuss in this chapter, which information can be gained and which experimental parameters need to be considered when planning such experiments. We focus especially on the interaction of diffusive ligands with membrane proteins.

Andrea Lakatos and Karsten Mörs contributed equally to this chapter.

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Authors and Affiliations

  1. Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University Frankfurt, Frankfurt, Germany

    Andrea Lakatos, Karsten Mörs & Clemens Glaubitz

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  1. Andrea Lakatos
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  2. Karsten Mörs
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  3. Clemens Glaubitz
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Correspondence to Clemens Glaubitz .

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Editors and Affiliations

  1. , Division of Immunology, Beckman Research Institute of the City o, E. Duarte Road 1500, Duarte, 91010, USA

    Nagarajan Vaidehi

  2. , Department of Structural Biology, University of Pittsburgh School of Medic, Fifth Avenue 3501, Pittsburgh, 15261, USA

    Judith Klein-Seetharaman

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Lakatos, A., Mörs, K., Glaubitz, C. (2012). How to Investigate Interactions Between Membrane Proteins and Ligands by Solid-State NMR. In: Vaidehi, N., Klein-Seetharaman, J. (eds) Membrane Protein Structure and Dynamics. Methods in Molecular Biology, vol 914. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-023-6_5

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  • DOI: https://doi.org/10.1007/978-1-62703-023-6_5

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