Abstract
The clinical potential of therapeutic quantities of primary hematopoietic cells, either unmodified or altered via genetic modification, has stimulated the search for techniques that allow the production of large numbers of hematopoietic precursors, more primitive progenitors, and perhaps hematopoietic stem cells (HSC) themselves. Modifications of in vitro culture conditions to promote progenitor cell expansion have included combinations of polypeptide cytokines, small molecules, and transcription factors. Here we describe the methods for use of the transcription factor linked to a TAT-based protein transcription domain, in combination with cytokines and serum-free culture condition to stimulate the proliferation of primary cells. Human peripheral blood (PB) CD34+ cells treated with TAT-NF-Ya fusion protein and grown in vitro for 1 month proliferate four times more than did cells in cultures that contained only cytokines, including increased production of hematopoietic cells of all maturities. These results and techniques should be suitable for multiple applications of ex vivo generation of hematopoietic cells using protein transduction.
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Acknowledgments
We are grateful to Steve Dowdy (University of California, San Diego) for kindly providing the pTAT-HA vector and Jiang Zhu (Shanghai Second Medical School, Shanghai, China) for the NF-Ya plasmid.
This study was supported by National Institute of Health (grant RO1-CA090833).
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Domashenko, A.D., Wiener, S., Emerson, S.G. (2012). NF-Ya Protein Delivery as a Tool for Hematopoietic Progenitor Cell Expansion. In: Mace, K., Braun, K. (eds) Progenitor Cells. Methods in Molecular Biology, vol 916. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-980-8_23
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DOI: https://doi.org/10.1007/978-1-61779-980-8_23
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