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Quantitative Mass Spectrometry-Based Proteomics: An Overview

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Quantitative Methods in Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 893))

Abstract

In recent years, mass spectrometry has moved more than ever before into the front line of protein-centered research. After being established at the qualitative level, the more challenging question of quantification of proteins and peptides using mass spectrometry has become a focus for further development. In this chapter, we discuss and review the strategies and problems of the methods currently in use for the quantitative analysis of peptides, proteins, and finally proteomes by mass spectrometry. The common themes, the differences, and the potential pitfalls of the main approaches are presented in order to provide a survey of the emerging field of quantitative, mass spectrometry-based proteomics.

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Author information

Authors and Affiliations

  1. Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany

    Miroslav Nikolov & Carla Schmidt

  2. Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Göttinger, Germany

    Henning Urlaub

Authors
  1. Miroslav Nikolov
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  2. Carla Schmidt
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  3. Henning Urlaub
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Corresponding author

Correspondence to Henning Urlaub .

Editor information

Editors and Affiliations

  1. Fak. Medizin, Medizinische Proteom Center, Universität Bochum, Universitätsstraße 150, Bochum, 44801, Germany

    Katrin Marcus

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Nikolov, M., Schmidt, C., Urlaub, H. (2012). Quantitative Mass Spectrometry-Based Proteomics: An Overview. In: Marcus, K. (eds) Quantitative Methods in Proteomics. Methods in Molecular Biology, vol 893. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-885-6_7

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  • DOI: https://doi.org/10.1007/978-1-61779-885-6_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-884-9

  • Online ISBN: 978-1-61779-885-6

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