Abstract
Next generation sequencing is a common and versatile tool for biological and medical research. We describe the basic steps for analyzing next generation sequencing data, including quality checking and mapping to a reference genome. We also explain the further data analysis for three common applications of next generation sequencing: variant detection, RNA-seq, and ChIP-seq.
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Gogol-Döring, A., Chen, W. (2012). An Overview of the Analysis of Next Generation Sequencing Data. In: Wang, J., Tan, A., Tian, T. (eds) Next Generation Microarray Bioinformatics. Methods in Molecular Biology, vol 802. Humana Press. https://doi.org/10.1007/978-1-61779-400-1_16
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DOI: https://doi.org/10.1007/978-1-61779-400-1_16
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