Abstract
Levels of telomerase activity can be an indicator of the proliferative potential of somatic cells and may serve as a diagnostic biomarker of malignancy. Telomeric repeat amplification protocol (TRAP) is a fast and sensitive PCR-based assay for detection and measurement of telomerase activity. Since its introduction, the TRAP assay has been widely used in cancer and aging studies. It provides a powerful alternative to other in vitro telomerase detection methods, and has been combined with other molecular techniques to screen telomerase inhibitors, and to study human malignancy and other diseases associated with telomere or telomerase dysfunction.
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References
Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, Coviello GM, Wright WE, Weinrich SL, Shay JW. (1994) Specific association of human telomerase activity with immortal cells and cancer. Science. 266 (5193):2011–  5.
Kim NW, Wu F. (1997) Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP). Nucleic Acids Res. 25(13):2595–7.
Burger AM. (2002) Standard TRAP assay. Methods Mol Biol. 191:109  –24.
Fajkus J. (2006) Detection of telomerase activity by the TRAP assay and its variants and alternatives. Clin Chim Acta. 371(1-2):25  –31.
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Xin, H. (2011). Telomeric Repeat Amplification Protocol: Measuring the Activity of the Telomerase. In: Songyang, Z. (eds) Telomeres and Telomerase. Methods in Molecular Biology, vol 735. Humana Press. https://doi.org/10.1007/978-1-61779-092-8_10
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DOI: https://doi.org/10.1007/978-1-61779-092-8_10
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Publisher Name: Humana Press
Print ISBN: 978-1-61779-091-1
Online ISBN: 978-1-61779-092-8
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