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Formalin-Fixed Paraffin-Embedded Tissues pp 161–180Cite as

Whole Genome Amplification of DNA Extracted from FFPE Tissues

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 724))

Abstract

Whole genome amplification systems were developed to meet the increasing research demands on DNA resources and to avoid DNA shortage. The technology enables amplification of nanogram amounts of DNA into microgram quantities and is increasingly used in the amplification of DNA from multiple origins such as blood, fresh frozen tissue, formalin-fixed paraffin-embedded tissues, saliva, buccal swabs, bacteria, and plant and animal sources. This chapter focuses on the use of GenomePlex® tissue Whole Genome Amplification Kit, to amplify DNA directly from archived tissue. In addition, this chapter documents our unique experience with the utilization of GenomePlex® amplified DNA using several molecular techniques including metaphase Comparative Genomic Hybridization, array Comparative Genomic Hybridization, and real-time quantitative polymerase chain reaction assays. GenomePlex® is a registered trademark of Rubicon Genomics Incorporation.

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Acknowledgments

This work is supported by Kuwait Foundation for the Advancement of Sciences grant number 2006-1302-07, Kuwait University Grant numbers YM10/07 and MG02/08, and Research Core Facility (RCF) grant number GM 01/01 and GM 01/05.

Author information

Authors and Affiliations

  1. Faculty of Medicine, Department of Pathology, Kuwait University, Safat, Kuwait

    Mira Bosso

  2. Molecular Pathology Unit, Health Sciences Center, Safat, Kuwait

    Fahd Al-Mulla

Authors
  1. Mira Bosso
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  2. Fahd Al-Mulla
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Corresponding author

Correspondence to Fahd Al-Mulla .

Editor information

Editors and Affiliations

  1. Health Science Center, Dept. of Pathology, Kuwait University, Jabriya, Block 3B, The Forth Ring Road, Safat, Kuwait

    Fahd Al-Mulla

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Bosso, M., Al-Mulla, F. (2011). Whole Genome Amplification of DNA Extracted from FFPE Tissues. In: Al-Mulla, F. (eds) Formalin-Fixed Paraffin-Embedded Tissues. Methods in Molecular Biology, vol 724. Humana Press. https://doi.org/10.1007/978-1-61779-055-3_11

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  • DOI: https://doi.org/10.1007/978-1-61779-055-3_11

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-054-6

  • Online ISBN: 978-1-61779-055-3

  • eBook Packages: Springer Protocols

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