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Argonaute Proteins pp 281–293Cite as

Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 725))

Abstract

Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs. We describe a method for the massive identification of piRNAs from immunopurified piRNPs. This strategy may also be used for immunopurification and directional sequencing of RNAs from other RNPs that contain small RNAs.

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Acknowledgment

Supported by NIH grants GM072777 and NS056070 to ZM.

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Authors and Affiliations

  1. Division of Neuropathology, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    Zissimos Mourelatos

Authors
  1. Yohei Kirino
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  2. Anastassios Vourekas
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  3. Eugene Khandros
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  4. Zissimos Mourelatos
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Corresponding author

Correspondence to Zissimos Mourelatos .

Editor information

Editors and Affiliations

  1. , Department of Cell Biology, University of Alberta, Edmonton, T6G 2H7, Alberta, Canada

    Tom C. Hobman

  2. Goodman Cancer Research Centre, Department of Biochemistry, McGill University, Avenue des Pins Ouest 1160, Montreal, H3A 1A3, Québec, Canada

    Thomas F. Duchaine

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Kirino, Y., Vourekas, A., Khandros, E., Mourelatos, Z. (2011). Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs. In: Hobman, T., Duchaine, T. (eds) Argonaute Proteins. Methods in Molecular Biology, vol 725. Humana Press. https://doi.org/10.1007/978-1-61779-046-1_18

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  • DOI: https://doi.org/10.1007/978-1-61779-046-1_18

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-045-4

  • Online ISBN: 978-1-61779-046-1

  • eBook Packages: Springer Protocols

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