Abstract
Desorption electrospray ionization mass spectrometry (DESI-MS) is a relatively new ambient surface analysis method. This technique requires little or no sample preparation prior to the actual analysis. Along with other application areas, DESI-MS is used to obtain chemical images of different biomolecules in biological tissue sections. In the case of biological tissue analysis, only preparation of the tissue slices is necessary. Furthermore, the method does not require the use of an ionization matrix preventing the redistribution of analytes prior to the analysis. Chemical images are obtained by monitoring the mass spectrometric signals of compounds while moving the sample relative to the DESI sprayer.
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References
Takáts, Z., Wiseman, J. M., Gologan, B., Cooks, R. G. (2004) Mass spectrometry sampling under ambient conditions with desorption electrospray ionization. Science, 306, 471–473.
Van Berkel, G. J., Pasilis S. P., Ovchinnikova, O. (2008) Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry. J Mass Spectrom, 43, 1161–1180.
Ifa, D. R., Wiseman, J. M., Song, Q., Cooks, R. G. (2007) Development of capabilities for imaging mass spectrometry under ambient conditions with desorption electrospray ionization (DESI). Int J Mass Spectrom, 259, 8–15.
Kertesz, V., Van Berkel, G. J. (2008) Scanning and surface alignment considerations in chemical imaging with desorption electrospray mass spectrometry. Anal Chem, 80, 1027–1032.
Ifa, D. R., Gumaelius, L. M., Eberlin, L. S., Manicke, N. E., Cooks, R. G. (2007) Forensic analysis of inks by imaging desorption electrospray ionization (DESI) mass spectrometry. Analyst, 132, 461–467.
Kertesz, V., Van Berkel, G. J. (2008) Improved imaging resolution in desorption electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom, 22, 2639–2644.
Van Berkel, G. J., Kertesz, V. (2006) Automated sampling and imaging of analytes separated on thin-layer chromatography plates using desorption electrospray ionization mass spectrometry. Anal Chem, 78, 4938–4944.
Pasilis, S. P., Kertesz, V., Van Berkel, G. J., Schulz, M., Schorcht, S. (2008) HPTLC/DESI-MS imaging of tryptic protein digests separated in two dimensions. J Mass Spectrom, 43, 1627–1635.
Ifa, D. R., Manicke, N. E., Dill, A. L., Cooks, R.G. (2008) Latent fingerprint chemical imaging by mass spectrometry. Science, 321, 805.
Wiseman, J. M., Ifa, D. R., Song Q., Cooks, R. G. (2006) Tissue imaging at atmospheric pressure using desorption electrospray ionization (DESI) mass spectrometry. Angew Chem Int Ed, 45, 7188–7192.
Wiseman, J. M., Ifa, D. R. Venter, A., Cooks, R. G. (2008) Ambient molecular imaging by desorption electrospray ionization mass spectrometry, Nat Protoc, 3, 517–524.
Wiseman, J. M., Ifa, D. R., Zhu, Y., Kissinger, C. B., Manicke, N. E., Kissinger, P. T., Cooks, R. G. (2008) Desorption electrospray ionization mass spectrometry: imaging drugs and metabolites in tissues. Proc Nat Acad Sci U S A, 105, 18120–18125.
Kertesz, V., Van Berkel, G. J., Vavrek, M., Koeplinger, K. A., Schneider, B. B., Covey, T. R. (2008) Comparison of drug distribution images from whole-body thin tissue sections obtained using desorption electrospray ionization tandem mass spectrometry and autoradiography. Anal Chem, 50, 5168–5177.
Leuthold, L. A., Mandscheff, J.-F., Fathi, M., Giroud, C., Augsburger, M., Varesio, E., Hopfgartner, G. (2006) Desorption electrospray ionization mass spectrometry: direct toxicological screening and analysis of illicit ecstasy tablets. Rapid Commun Mass Spectrom, 20, 103–110.
Pasilis, S. P., Kertesz, V., Van Berkel, G. J. (2008) Surface scanning analysis of planar arrays of analytes with desorption electrospray ionization-mass spectrometry. Anal Chem, 79, 5956–5962.
Acknowledgments
The imaging protocols described here were derived from work supported by the Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences, United States Department of Energy under Contract DE-AC05-00OR22725 with ORNL, managed and operated by UT-Battelle, LLC.
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Kertesz, V., Van Berkel, G.J. (2010). Chemical Imaging with Desorption Electrospray Ionization Mass Spectrometry. In: Rubakhin, S., Sweedler, J. (eds) Mass Spectrometry Imaging. Methods in Molecular Biology, vol 656. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-746-4_13
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DOI: https://doi.org/10.1007/978-1-60761-746-4_13
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